Induction of triploidy with caffeine treatment in the African catfish (Clarias gariepinus)

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dc.contributor.authorTuran, F.
dc.contributor.authorGuragac, R.
dc.date.accessioned2024-09-18T20:11:29Z
dc.date.available2024-09-18T20:11:29Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractInduction of triploidy is one of the biotechnological methods in aquaculture used for genetic manipulation. It refers to a state where organisms have three complete sets of chromosomes instead of two and can result in sterility. Caffeine treatment that is safe and inexpensive, serve to induce triploidy in catfish. To suppress the second meiotic division, fertilized eggs were exposed to three different concentrations (5, 10 and 15 mM) of caffeine solution for 20 min beginning at 3 min after fertilization. After that, the eggs were incubated at ambient temperature until hatching. The induction of triploidy in fry was determined for three concentrations of caffeine by means of flow-cytometric analysis. The lowest rate of triploidy (20.40 +/- 1.13%) was obtained in the group treated with 5 mM caffeine and the highest (69.10 +/- 2.18%) in the group treated with 15 mM caffeine. Our results suggest that caffeine can be used to induce triploidy in catfish.en_US
dc.description.sponsorshipUniversity of Mustafa Kemal in Turkey [0204 Y 0102]; Izmir Institute of Technology (in Turkey)en_US
dc.description.sponsorshipThe study was supported by the project 0204 Y 0102 of University of Mustafa Kemal in Turkey. Also, the authors gratefully acknowledge the support of Izmir Institute of Technology (in Turkey) for the use of the Institute laboratory.en_US
dc.identifier.endpage1020en_US
dc.identifier.issn1562-2916
dc.identifier.issue4en_US
dc.identifier.startpage1014en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8888
dc.identifier.volume13en_US
dc.identifier.wosWOS:000353485300018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherIranian Fisheries Science Research Inst-Ifsrien_US
dc.relation.ispartofIranian Journal of Fisheries Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClarias gariepinusen_US
dc.subjectTriploidyen_US
dc.subjectCaffeineen_US
dc.subjectFlow cytometryen_US
dc.titleInduction of triploidy with caffeine treatment in the African catfish (Clarias gariepinus)en_US
dc.typeArticleen_US

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