Different fosA genes were found on mobile genetic elements in Escherichia coli from wastewaters of hospitals and municipals in Turkey

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dc.authoridHammerl, Jens Andre/0000-0002-6930-4358
dc.authoridStingl, Kerstin/0000-0002-8338-717X
dc.contributor.authorGuneri, Cansu Onlen
dc.contributor.authorStingl, Kerstin
dc.contributor.authorGrobbel, Mirjam
dc.contributor.authorHammerl, Jens Andre
dc.contributor.authorKurekci, Cemil
dc.date.accessioned2024-09-18T20:25:12Z
dc.date.available2024-09-18T20:25:12Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAims: The increasing number of globally established fosfomycin-resistant (FosR) Gram-negative bacteria inspired us to investigate the occurrence of FosR Enterobacterales populations (esp. E. coli) in samples of city wastewater treatment plants (WWTPs) and hospital sewage in Hatay, Turkey. FosR target bacteria were further characterized for their clonal relatedness, resistomes and mobile genetic elements (MGEs) to evaluate their impact on fosfomycin resistance dissemination. Methods: A total of 44 samples from raw and treated waters of WWTPs as well as of two hospitals in the Hatay province were subjected to selective cultivation for recovering FosR Enterobacterales. The presence of fosA was verified by PCR and Sanger amplicon sequencing. Detected E. coli were further evaluated against antimicrobial susceptibility-testing, macrorestriction profiling (PFGE) and whole-genome sequencing (WGS). Bioinformatics analysis was performed for genome subtyping (i.e., MLST, serotype), resistome/virulome determination and dissection of the genetic determinants of plasmidic fosA3/4 resistances. Results: Besides ten non-E. coli Enterobacterales, 29 E. coli were collected within this study. In silico-based subtyping revealed that E. coli isolates were assigned to six different serovars and 14 sequence types (ST), while O8:H21 and ST410 represented the major prevalent types, respectively. Fosfomycin resistance in the isolates was found to be mediated by the fosA4 (n = 18), fosA3 (n = 10) and fosA (n = 1), which are frequently associated with transmissible MGEs. Reconstruction of plasmid-associated fosA gene context revealed a linkage between the resistance cassette and IS6 (IS26 family) transposases, which might represent a major driver for the distribution of the genes and the generation of novel fosA-carrying plasmids. Conclusions: The occurrence of plasmid-mediated, transmissible FosR in E. coli from wastewater pose a foreseeable threat to One-Health. To minimize further spread of the resistances in bacterial populations associated with environmental, animal and human health further resistance monitoring and management strategies must be developed.en_US
dc.description.sponsorshipHatay Mustafa Kemal University [BAP 18.M.032]; Werner Baltes fellowship; BfR [43-001, 1322-648]; European Joint Programme (project: ARDIG); European Joint Programme (project: FULL_FORCE); German Federal Ministry for Health (projects: GUCCI)en_US
dc.description.sponsorshipThis project was partially founded by Hatay Mustafa Kemal University (BAP 18.M.032). C. K. was a recipient of Werner Baltes fellowship. The work of J.A.H was supported by grants of the BfR (43-001, 1322-648), the European Joint Programme (projects: ARDIG and FULL_FORCE) and the German Federal Ministry for Health (projects: GUCCI). The authors declare no conflict of interest. The support of PlasmidID analysis by Katharina Juraschek is also highly acknowledged. We also gratefully thank Assoc. Prof. Dr. Errol Hassan (The University of Queensland) for critical review of the manuscript.en_US
dc.identifier.doi10.1016/j.scitotenv.2022.153928
dc.identifier.issn0048-9697
dc.identifier.issn1879-1026
dc.identifier.pmid35182630en_US
dc.identifier.scopus2-s2.0-85124935946en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.scitotenv.2022.153928
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10151
dc.identifier.volume824en_US
dc.identifier.wosWOS:000764890800004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofScience of The Total Environmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFosfomycin resistanceen_US
dc.subjectEnterobacteralesen_US
dc.subjectWastewateren_US
dc.subjectPlasmiden_US
dc.subjectTransmissionen_US
dc.titleDifferent fosA genes were found on mobile genetic elements in Escherichia coli from wastewaters of hospitals and municipals in Turkeyen_US
dc.typeArticleen_US

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