Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus
dc.authorid | Comelekoglu, Ulku/0000-0001-8060-6333 | |
dc.authorid | Mete, Turkan/0000-0002-4159-1965 | |
dc.contributor.author | Arpaci, Abdullah | |
dc.contributor.author | Yalin, Serap | |
dc.contributor.author | Ecevit, Hasret | |
dc.contributor.author | Comelekoglu, Ulku | |
dc.contributor.author | Mete, Turkan | |
dc.date.accessioned | 2024-09-18T20:15:09Z | |
dc.date.available | 2024-09-18T20:15:09Z | |
dc.date.issued | 2020 | |
dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
dc.description.abstract | Background. Diabetes mellitus (DM) has become more and more common and has a high morbidity and mortality rate worldwide. It is a multifactorial chronic disease affected by both genetic and environmental factors. Objectives. To evaluate the association between antioxidant enzyme activities and their genetic variations and the level of malondialdehyde (MDA) in type II diabetes patients living in the Adlyaman province in the southeast part of Turkey. Material and methods. One hundred patients diagnosed with type II DM (T2DM) and 100 healthy controls were included in the study. Malondialdehyde levels and antioxidant enzyme activities were measured spectrophometrically. DNA isolation was performed and genotyping was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. Our results revealed no significant differences in genotype distributions and allele frequencies of all polymorphisms between groups (p > 0.05). Significantly elevated MDA levels and a significant reduction in catalase (CAT) and paraoxonase (PON) enzyme activities were observed in patients compared to the control group in terms of study groups and genetic variations (p < 0.05). Moreover, CAT activity was reduced in TT genotype in terms of CAT -262 C/T polymorphism in patients (p < 0.05). Paraoxonase activity was observed to be lower in MM genotype in both groups (p < 0.05). Conclusions. CAT-262 C/T polymorphism may be one of the factors that lead to severe clinical situation in DM. Our results suggest that TT genotype may be more prone to lipid peroxidation. | en_US |
dc.description.sponsorship | Scientific Research Projects Commission of Adiyaman University, Turkey; TIPFBAP [2010/0001] | en_US |
dc.description.sponsorship | This study was supported with a grant from Scientific Research Projects Commission of Adiyaman University, Turkey (grant No. TIPFBAP 2010/0001). | en_US |
dc.identifier.doi | 10.17219/acem/125397 | |
dc.identifier.endpage | 1063 | en_US |
dc.identifier.issn | 1899-5276 | |
dc.identifier.issn | 2451-2680 | |
dc.identifier.issue | 9 | en_US |
dc.identifier.pmid | 32926601 | en_US |
dc.identifier.scopus | 2-s2.0-85092411327 | en_US |
dc.identifier.scopusquality | Q1 | en_US |
dc.identifier.startpage | 1057 | en_US |
dc.identifier.uri | https://doi.org/10.17219/acem/125397 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12483/9476 | |
dc.identifier.volume | 29 | en_US |
dc.identifier.wos | WOS:000577257100005 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wroclaw Medical Univ | en_US |
dc.relation.ispartof | Advances in Clinical and Experimental Medicine | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | diabetes mellitus | en_US |
dc.subject | oxidative stress | en_US |
dc.subject | cat | en_US |
dc.subject | malondialdehyde | en_US |
dc.subject | paraoxonase | en_US |
dc.title | Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus | en_US |
dc.type | Article | en_US |