Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus

dc.authoridComelekoglu, Ulku/0000-0001-8060-6333
dc.authoridMete, Turkan/0000-0002-4159-1965
dc.contributor.authorArpaci, Abdullah
dc.contributor.authorYalin, Serap
dc.contributor.authorEcevit, Hasret
dc.contributor.authorComelekoglu, Ulku
dc.contributor.authorMete, Turkan
dc.date.accessioned2024-09-18T20:15:09Z
dc.date.available2024-09-18T20:15:09Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground. Diabetes mellitus (DM) has become more and more common and has a high morbidity and mortality rate worldwide. It is a multifactorial chronic disease affected by both genetic and environmental factors. Objectives. To evaluate the association between antioxidant enzyme activities and their genetic variations and the level of malondialdehyde (MDA) in type II diabetes patients living in the Adlyaman province in the southeast part of Turkey. Material and methods. One hundred patients diagnosed with type II DM (T2DM) and 100 healthy controls were included in the study. Malondialdehyde levels and antioxidant enzyme activities were measured spectrophometrically. DNA isolation was performed and genotyping was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. Our results revealed no significant differences in genotype distributions and allele frequencies of all polymorphisms between groups (p > 0.05). Significantly elevated MDA levels and a significant reduction in catalase (CAT) and paraoxonase (PON) enzyme activities were observed in patients compared to the control group in terms of study groups and genetic variations (p < 0.05). Moreover, CAT activity was reduced in TT genotype in terms of CAT -262 C/T polymorphism in patients (p < 0.05). Paraoxonase activity was observed to be lower in MM genotype in both groups (p < 0.05). Conclusions. CAT-262 C/T polymorphism may be one of the factors that lead to severe clinical situation in DM. Our results suggest that TT genotype may be more prone to lipid peroxidation.en_US
dc.description.sponsorshipScientific Research Projects Commission of Adiyaman University, Turkey; TIPFBAP [2010/0001]en_US
dc.description.sponsorshipThis study was supported with a grant from Scientific Research Projects Commission of Adiyaman University, Turkey (grant No. TIPFBAP 2010/0001).en_US
dc.identifier.doi10.17219/acem/125397
dc.identifier.endpage1063en_US
dc.identifier.issn1899-5276
dc.identifier.issn2451-2680
dc.identifier.issue9en_US
dc.identifier.pmid32926601en_US
dc.identifier.scopus2-s2.0-85092411327en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1057en_US
dc.identifier.urihttps://doi.org/10.17219/acem/125397
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9476
dc.identifier.volume29en_US
dc.identifier.wosWOS:000577257100005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWroclaw Medical Univen_US
dc.relation.ispartofAdvances in Clinical and Experimental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectdiabetes mellitusen_US
dc.subjectoxidative stressen_US
dc.subjectcaten_US
dc.subjectmalondialdehydeen_US
dc.subjectparaoxonaseen_US
dc.titleEnzyme activity and genetic polymorphisms in patients with type II diabetes mellitusen_US
dc.typeArticleen_US

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