Purification and characterization of nitric oxide synthase from bovine kidney and investigating drug-induced toxicities of some antibiotics on the enzyme activity

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dc.authorscopusid36247613400
dc.authorscopusid23027537500
dc.authorscopusid6603903192
dc.contributor.authorIşgör, Mehmet Mustafa
dc.contributor.authorEkinci, Deniz
dc.contributor.authorBeydemir, Şükrü
dc.date.accessioned2024-09-19T15:43:32Z
dc.date.available2024-09-19T15:43:32Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIntroduction: Broad spectrums of antibiotics are widely used in the treatment of bacterial infections, particularly causing Staphylococcus aureus. These drugs have high acute renal injury (AKI) potency due to an increase in nitric oxide level. In this study, potency of nitric oxide synthase inhibition of some antibiotics was investigated according to vancomycin which was used as a reference antibiotic in renal injury. Methods and Results: Nitric oxide synthase (NOS) enzyme was purified by using 2'5'-ADP Sepharose 4B affinity chromatography from bovine kidney tissue in a single step with a yield of 3.58% and 1217-fold. Native and subunit molecular weights of the purified kidney NOS enzyme were calculated as 215 kDa and 114.8 kDa. Optimum pH, optimum ionic strength, optimum temperature and stable pH values for purified enzyme were determined as 7.5, 50 mM, 10°C and a pH range of 5.5-6.5, respectively. In vitro effects of antibiotics on purified enzyme activity were investigated by drawing Lineweaver-Burk plots. Enrofloxacin, kanamycin, chloramphenicol, gentamicin, vancomycin, cefazolin, streptomycin and ampicillin inhibited enzyme activity. IC50 values of these compounds were determined as 0.189 mM, 0.295 mM, 1.509 mM, 6.614 mM, 16.579 mM, 18.679 mM, 28.171 mM and 30.394 mM, respectively. Conclusion: Enrofloxacin and kanamycin were observed to have stronger NOS inhibitory effects as compared to vancomycin and may be more reliable antibiotics for use in renal infections as an alternative to vancomycin. © 2017 Bentham Science Publishers.en_US
dc.description.sponsorshipAtaturk University; University Research Fund Accounting, (2011/88)en_US
dc.identifier.doi10.2174/1573408013666170213152316
dc.identifier.endpage215en_US
dc.identifier.issn1573-4080
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85032898943en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage210en_US
dc.identifier.urihttps://doi.org/10.2174/1573408013666170213152316
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14391
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBentham Science Publishers B.V.en_US
dc.relation.ispartofCurrent Enzyme Inhibitionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibioticsen_US
dc.subjectInhibitionen_US
dc.subjectNitric oxide synthaseen_US
dc.subjectRenal injuryen_US
dc.subjectToxicitiesen_US
dc.titlePurification and characterization of nitric oxide synthase from bovine kidney and investigating drug-induced toxicities of some antibiotics on the enzyme activityen_US
dc.typeArticleen_US

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