Antioxidant effects of oleuropein on hydrogen peroxide-induced neuronal stress-an in vitro study

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dc.authorscopusid24481574200
dc.authorscopusid36247613400
dc.authorscopusid16229987500
dc.authorscopusid57215211660
dc.authorscopusid57194427293
dc.contributor.authorKucukgul, Altug
dc.contributor.authorIsgor, Mehmet M.
dc.contributor.authorDuzguner, Vesile
dc.contributor.authorAtabay, Meryem N.
dc.contributor.authorKucukgul, Azime
dc.date.accessioned2024-09-19T15:43:32Z
dc.date.available2024-09-19T15:43:32Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Persistent oxidative stress can lead to chronic inflammation and mediate most chronic diseases including neurological disorders. Oleuropein has been shown to be a potent antioxidant molecule in olive oil leaf having antioxidative properties. Objective: The aim of this study was to investigate the protective effects of oleuropein against oxidative stress in human glioblastoma cells. Methods: Human glioblastoma cells (U87) were pretreated with oleuropein (OP) essential oil 10 µM. After 30 minutes, 100 µM H2 O2 was added to the cells for three hours. Cell survival was quantified by colorimetric MTT assay. Glutathione level, total oxidant capacity, total antioxidant capacity and nitric oxide levels were determined by using specific spectrophotometric methods. The relative gene expression level of iNOS was performed by qRT-PCR method. Results: According to viability results, the effective concentration of H2 O2 (100µM) significantly decreased cell viability and oleuropein pretreatment significantly prevented the cell losses. Oleuropein regenerated total antioxidant capacity and glutathione levels decreased by H2 O2 exposure. In addition, nitric oxide and total oxidant capacity levels were also decreased after administration of oleuropein in treated cells. Conclusion: Oleuropein was found to have potent antioxidative properties in human glioblastoma cells. However, further studies and validations are needed in order to understand the exact neuroprotective mechanism of oleuropein. © 2020 Bentham Science Publishers.en_US
dc.identifier.doi10.2174/1871523018666190201145824
dc.identifier.endpage82en_US
dc.identifier.issn1871-5230
dc.identifier.issue1en_US
dc.identifier.pmid30714532en_US
dc.identifier.scopus2-s2.0-85068918104en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage72en_US
dc.identifier.urihttps://doi.org/10.2174/1871523018666190201145824
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14401
dc.identifier.volume19en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publishersen_US
dc.relation.ispartofAnti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnti-inflammatoryen_US
dc.subjectAnti-oxidanten_US
dc.subjectGlioblastomaen_US
dc.subjectIn vitroen_US
dc.subjectNeuroprotectionen_US
dc.subjectOleuropeinen_US
dc.titleAntioxidant effects of oleuropein on hydrogen peroxide-induced neuronal stress-an in vitro studyen_US
dc.typeArticleen_US

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