The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa

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dc.authoridKocak, Gokhan/0000-0003-1917-9090
dc.contributor.authorKocak, Gokhan
dc.contributor.authorYildiz, Cengiz
dc.date.accessioned2024-09-18T20:32:58Z
dc.date.available2024-09-18T20:32:58Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose + 3% skimmed milk powder sperm extender on the freezing of mouse spermatozoa in liquid nitrogen were investigated. The combination of 18% raffinose + 3% skimmed milk powder without additives was used as the control group. Frozen spermatozoa were thawed in a 37 degrees C water bath for 30 seconds. After freeze-thawing, motility, dead spermatozoa ratio, plasma membrane integrity, abnormal acrosome ratio, motility endurance (for 4 hours), and cell apoptosis tests were performed in Human Tubal Fluid (HTF). Compared with the control group after freezing and thawing, the highest motility and plasma membrane integrity were obtained in the 10 mM L-glutamine group with 56.6% +/- 2.11% and 77.8% +/- 0.87%, respectively (p < 0.05). In addition, when compared to the control group, the lowest rate of dead spermatozoa and abnormal acrosome was found in the 10 mM L-glutamine group as 26.0% +/- 1.46% and 6.3% +/- 1.09%, respectively (p < 0.05). The highest motility values for spermatozoa endurance were determined in the 10 and 50 mM L-glutamine groups up to the 4th hour compared to the control group (p < 0.05). In the evaluation of apoptosis in semen samples, there was no significant difference between the control, 0.1 mM ferulic acid, and 10 mM L-glutamine groups (p > 0.05). As a result, it was determined that the addition of 10 mM L-glutamine to the spermatozoa extender increased the motility, viable spermatozoa, functional membrane integrity, intact acrosome ratios, or motility endurance after freeze-thawing and could be used successfully in the freezing extender of mouse spermatozoa.en_US
dc.description.sponsorshipCommission of Scientific Researches Projects [21.D.001]; Hatay Mustafa Kemal University, Turkeyen_US
dc.description.sponsorshipThis study was supported by the Commission of Scientific Researches Projects (Project number: 21.D.001), the Hatay Mustafa Kemal University, Turkey.en_US
dc.identifier.doi10.1089/bio.2023.0067
dc.identifier.endpage293en_US
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.issue3en_US
dc.identifier.pmid38150493en_US
dc.identifier.scopus2-s2.0-85182663207en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage286en_US
dc.identifier.urihttps://doi.org/10.1089/bio.2023.0067
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11244
dc.identifier.volume22en_US
dc.identifier.wosWOS:001152595800001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofBiopreservation and Biobankingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmouseen_US
dc.subjectfrozen-thawed semenen_US
dc.subjectferulic aciden_US
dc.subjecttryptophanen_US
dc.subjectL-glutamineen_US
dc.titleThe Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoaen_US
dc.typeArticleen_US

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