Comparative Evaluation of Transgenic and Non Transgenic Tobacco for the Phytoextraction of Nickel-Contaminated Soils
Yükleniyor...
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Foundation Environmental Protection & Research-Fepr
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Nickel (Ni) accumulation ability and phytoextraction potential of Nicotiana tabacum L. cv Petit Havana SR-1 and Metallothionein II (MTII) gene transferred transgenic plants (p-S-ScMTII) of the same cultivar were grown in hydroponic culture containing 0, 5 and 10 mg L-1 Ni for 14 days. The effects of increasing Ni concentrations on plant dry matter production, Ni accumulation, chlorophyll and glutathione concentration were investigated. Moderate wilting and wrinkling were observed upon Ni treatments at the early stage of the growth, these symptoms severely increased at the harvest in both plant types. In addition, poor root growth and dark brown colour were observed. Dry matter content of transgenic tobacco was lower than that of the non-transgenic one. With the increasing Ni doses, Ni concentration, chlorophyll level and -SH (glutathione) concentration increased. Results showed that transgenic tobacco (128 mg kg(-1)) accumulated about 1.47-fold more Ni than that of non-transgenic one in shoots (87 mg kg-1) at 10 mg L-1 Ni treatment. Consequently, transfering the MTII gene into the plants to be used for phytoextraction may be regarded as promissing in improving Ni accumulation ability of plants.
Açıklama
Anahtar Kelimeler
Metallothionein, nickel, phytoextraction, transgenic tobacco
Kaynak
Ekoloji
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
21
Sayı
84
