Prevalence and molecular characterization of Listeria monocytogenes isolated from wastewater of cattle slaughterhouses in Turkey

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dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridDisli, Huseyin Burak/0000-0001-8255-3802
dc.authoridERTAS ONMAZ, Nurhan/0000-0002-4679-6548
dc.authoridAL, Serhat/0000-0003-2721-9275
dc.contributor.authorAl, Serhat
dc.contributor.authorDisli, Huseyin Burak
dc.contributor.authorHizlisoy, Harun
dc.contributor.authorErtas Onmaz, Nurhan
dc.contributor.authorYildirim, Yeliz
dc.contributor.authorGonulalan, Zafer
dc.date.accessioned2024-09-18T20:29:36Z
dc.date.available2024-09-18T20:29:36Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim The study aimed to investigate the role of cattle slaughterhouse wastewater as a possible source for the environmental distribution of Listeria monocytogenes. Methods and Results Listeria spp. isolation was performed by collecting 117 wastewater samples from four different cattle slaughterhouses in Turkey. Species-specific identification was performed biochemically, and L. monocytogenes isolates were confirmed with polymerase chain reaction (PCR). In all, 71 (62.2%) of the wastewater samples were found to be positive for Listeria spp., and 17 (14.9%) of these samples were contaminated with L. monocytogenes. Pulsed field gel electrophoresis (PFGE) analysis revealed that all L. monocytogenes isolates were of different pulsotypes and isolates belonged to seven different phylogenetic clusters. Multiplex PCR analysis for genoserotypes and lineage determination showed that the isolates were divided into genoserotypes IVb and IIc in Lineages I and II. Also, it has been investigated with SYBR-Green Real-time PCR whether the L. monocytogenes isolates harboured virulence genes (hly, sigB, plcA, plcB, inlA, inlB, inlC and inlJ), and it was found that all isolates were substantially positive. Antibiotic resistance profiles and MIC values of the isolates were determined, and all L. monocytogenes isolates were found susceptible to ampicillin. In contrast, two isolates were resistant to meropenem and erythromycin, and three isolates were resistant to trimethoprim/sulfamethoxazole. Conclusion L. monocytogenes, which pose a threat to public health and resists to antibiotics effectively used in treatments, can environmentally spread via wastewater of cattle slaughterhouses. The wastewater of the food industry, which has rich microbiota, should be treated carefully, and possible environmental contamination should be prevented. Significance and Impact of Study This is the first study that investigates the molecular characterization of L. monocytogenes isolated from cattle slaughterhouse wastewater and the findings represent the importance of cattle wastewater in the epidemiology of L. monocytogenes in Turkey.en_US
dc.description.sponsorshipErciyes University, Scientific Research Project Coordination Unit [THD-2018-8061]en_US
dc.description.sponsorshipThis study was supported by Erciyes University, Scientific Research Project Coordination Unit with project number THD-2018-8061.en_US
dc.identifier.doi10.1111/jam.15261
dc.identifier.endpage1525en_US
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.issue2en_US
dc.identifier.pmid34415644en_US
dc.identifier.scopus2-s2.0-85113371137en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1518en_US
dc.identifier.urihttps://doi.org/10.1111/jam.15261
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10962
dc.identifier.volume132en_US
dc.identifier.wosWOS:000688562200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofJournal of Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectListeriaen_US
dc.subjectmolecular epidemiologyen_US
dc.subjectresistanceen_US
dc.subjectvirulenceen_US
dc.subjectwastewateren_US
dc.titlePrevalence and molecular characterization of Listeria monocytogenes isolated from wastewater of cattle slaughterhouses in Turkeyen_US
dc.typeArticleen_US

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