The Effects of Different Extenders and Myo-Inositol on Post-thaw Quality of Ram Semen

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dc.authoridAkcay, Ergun/0000-0002-7491-5671
dc.authoridATESSAHIN, Ahmet/0000-0002-1004-2146
dc.contributor.authorKulaksiz, Recai
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorAkcay, Ergun
dc.contributor.authorSakin, Fatih
dc.contributor.authorDaskin, Ali
dc.contributor.authorAtessahin, Ahmet
dc.date.accessioned2024-09-18T20:55:38Z
dc.date.available2024-09-18T20:55:38Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractThe study was conducted to evaluate the effects of different extenders and inositol additions on post-thaw semen quality, lipid peroxidation (LPO) and antioxidant activities. Semen was collected from four Karayaka rams from by artificial vagina three times a week. Semen samples showing normospermy quality were pooled. The pooled semen samples were extended in three extenders (Tris, T-, skimmed milk, M-and sodium citrate, NaC) with myo-inositol at two different doses (5 mM, 10 mM) and no antioxidant (control). Nine experimental groups were assigned as follows: T-5I, T-10I, T (control); M-5I, M-10I, M (control); Na-5I, Na-10I, NaC (control). Straws containing extended semen were equilibrated at 4 degrees C for 2 h, frozen in vapor of (15 min at -120 degrees C) liquid nitrogen and stored in liquid nitrogen. Frozen semen was thawed in a water bath at 37 degrees C for 30 seconds. The use of all the extenders supplemented with different doses of myo-inositol did not lead to any significant improvement in microscopic sperm and oxidative stress parameters (P > 0.05). Extenders of T and M resulted in higher sperm motility (50.00+/-2.24% and 55.00+/-0.42%) and HOST (49.00+/-3.32% and 48.17+/-2.97%) rates, compared to NaC (37.00+/-3.74% and 31.80+/-2.96%, P < 0.01), following the freeze/thawing process. Extenders supplementated with myo-inositol not significantly affect malondialdehyde (MDA) levels and activities of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) in comparison to the control groups (P > 0.05), except for MDA level of T extender containing 10 mM inositol. MDA level was found lower (1.22+/-0.07 nmol/ml) in T than those of the M and NaC (P < 0.05). For GSH and GSH-PX activities, T and NaC gave the higher values, compared to M, following the freeze/thawing process (P < 0.01).en_US
dc.description.sponsorshipTUBITAK, Turkey [KAMAG-106G005]en_US
dc.description.sponsorshipThis study was supported by grants from TUBITAK, Turkey (KAMAG-106G005)en_US
dc.identifier.endpage222en_US
dc.identifier.issn1300-6045
dc.identifier.issue2en_US
dc.identifier.startpage217en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11973
dc.identifier.volume17en_US
dc.identifier.wosWOS:000288117600009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRam semenen_US
dc.subjectInositolen_US
dc.subjectCryopreservationen_US
dc.subjectSemen parametersen_US
dc.subjectAntioxidant activitiesen_US
dc.titleThe Effects of Different Extenders and Myo-Inositol on Post-thaw Quality of Ram Semenen_US
dc.typeArticleen_US

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