Thermostable alkaline protease produced by an Aspergillus niger strain

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dc.authoridCoral, Gokhan/0000-0002-3533-118X
dc.contributor.authorCoral, G
dc.contributor.authorArikan, B
dc.contributor.authorÜnaldi, MN
dc.contributor.authorGüvenmez, H
dc.date.accessioned2024-09-18T19:52:35Z
dc.date.available2024-09-18T19:52:35Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractA thermostable alkaline protease was isolated from the Aspergillus niger ZI strain in a liquid Czapek Dox medium, containing casein (1% w/v) as the sole nitrogen source. Enzyme extract was subjected to electrophoresis in SDS-polyacrylamide gel. Two protein bands were seen on polyacrylamide gel. Active enzyme was visualized in a zymograin and protease activity exhibited a molecular mass of 68 kDa on SDS-polyacrylamide gel. The optimum pH for activity was found to be 9.0. The temperature optima of the enzyme was found to be 40 degreesC at pH 9.0 and it remained stable up to 90 degreesC, with 48.4% of the original activity retained after heat treatment at 90 degreesC for 15 min. Proteolytic activity was inhibited by PMSF, but slightly inhibited by SDS.en_US
dc.identifier.endpage498en_US
dc.identifier.issn1590-4261
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0347659337en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage491en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7534
dc.identifier.volume53en_US
dc.identifier.wosWOS:000187783400008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Microbiologiaen_US
dc.relation.ispartofAnnals of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalkaline proteaseen_US
dc.subjectAspergillus nigeren_US
dc.titleThermostable alkaline protease produced by an Aspergillus niger strainen_US
dc.typeArticleen_US

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