The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR

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dc.contributor.authorOnlen, Cansu
dc.contributor.authorDuran, Nizami
dc.contributor.authorBayraktar, Suphi
dc.contributor.authorAy, Emrah
dc.contributor.authorOzer, Burcin
dc.date.accessioned2024-09-18T20:16:36Z
dc.date.available2024-09-18T20:16:36Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim: The aim of the present study was to determine the frequency of shiga-like toxin (stx1 and stx2) and drug resistance profiles food-borne Escherichia coli O157: H7 in Hatay province, Turkey. Methods: The presence of the virulence genes (stx1, stx2, hlyA) in a total of 150 E. coli isolates were studied with multiplex PCR. Results: A total of 327 salad samples were analyzed. E. coli O157: H7 was detected in 150 (45.8 %) out of 327 analyzed samples. Of these 150 isolates, the presence of hly-A gene was detected in 32 (21.3%) E. coli isolates. A total of five (15.6%) isolates in this 32 hlyA positive isolates had stx2 gene, two (6.3%) of them had stx1 gene and one (3.1%) of the isolates was found to be positive for both stx1 and stx2 genes. It was found that all E. coli O157: H7 isolates were resistant to erythromycin. While the highest rate of antibiotic resistance was observed for ampicillin (68.8%), no antibiotic resistance against cefuroxime, ciprofloxacin and cephaperasone was identified. Conclusions: The results obtained in our province showed that E. coli strains isolated from salad samples were found to have some important virulence genes such as stx1, stx2, and hlyA. The stx2 frequency was found to be higher than stx1 frequency. Also, it was observed that there was not any significant correlation between drug resistance profiles and presence of toxin genes in E. coli O157: H7 strains. As a result, increasing frequency of STEC O157 serotype among foodborne pathogens is a growing public health problem.en_US
dc.description.sponsorshipMustafa Kemal Universityen_US
dc.description.sponsorshipThis work was supported by the Research Fund of Mustafa Kemal University. This study has been in accordance with both legal and ethical grounds.en_US
dc.identifier.doi10.1515/rrlm-2017-0025
dc.identifier.endpage326en_US
dc.identifier.issn1841-6624
dc.identifier.issn2284-5623
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85032668801en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage317en_US
dc.identifier.urihttps://doi.org/10.1515/rrlm-2017-0025
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9624
dc.identifier.volume25en_US
dc.identifier.wosWOS:000417164200001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Pressen_US
dc.relation.ispartofRevista Romana De Medicina De Laboratoren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectE.coli O157: H7en_US
dc.subjectstx1en_US
dc.subjectstx2en_US
dc.subjecthly-Aen_US
dc.subjectPCRen_US
dc.titleThe frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCRen_US
dc.typeArticleen_US

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