Investigation of key miRNAs and target genes in bladder cancer using miRNA profiling and bioinformatic tools

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dc.authoridOzen, Mustafa/0000-0002-6142-5294
dc.contributor.authorCanturk, Kemal Murat
dc.contributor.authorOzdemir, Muhsin
dc.contributor.authorCan, Cavit
dc.contributor.authorOner, Setenay
dc.contributor.authorEmre, Ramazan
dc.contributor.authorAslan, Huseyin
dc.contributor.authorCilingir, Oguz
dc.date.accessioned2024-09-18T20:04:33Z
dc.date.available2024-09-18T20:04:33Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractDespite the association of several miRNAs with bladder cancer, little is known about the miRNAs' regulatory networks. In this study, we aimed to construct potential networks of bladder-cancer-related miRNAs and their known target genes using miRNA expression profiling and bioinformatics tools and to investigate potential key molecules that might play roles in bladder cancer regulatory networks. Global miRNA expression profiles were obtained using microarray followed by RT-qPCR validation using two randomly selected miRNAs. Known targets of deregulated miRNAs were utilized using DIANA-TarBase database v6.0. The incorporation of deregulated miRNAs and target genes into KEGG pathways were utilized using DIANA-mirPath software. To construct potential miRNA regulatory networks, the overlapping parts of three selected KEGG pathways were visualized by Cytoscape software. We finally gained 19 deregulated miRNAs, including 5 ups-and 14 down regulated in 27 bladder-cancer tissue samples and 8 normal urothelial tissue samples. The enrichment results of deregulated miRNAs and known target genes showed that most pathways were related to cancer or cell signaling pathways. We determined the hub CDK6, BCL2, E2F3, PTEN, MYC, RB, and ERBB3 target genes and hub hsa-let-7c, hsa-miR-195-5p, hsa-miR-141-3p, hsa-miR-26a-5p, hsa-miR-23b-3p, and hsa-miR-125b-5p miRNAs of the constructed networks. These findings provide new insights into the bladder cancer regulatory networks and give us a hypothesis that hsa-let-7c, hsa-miR-195-5p, and hsa-miR-125b-5p, along with CDK4 and CDK6 genes might exist in the same bladder cancer pathway. Particularly, hub miRNAs and genes might be potential biomarkers for bladder cancer clinics.en_US
dc.description.sponsorshipEskisehir Osmangazi University Research Fund [200811031]en_US
dc.description.sponsorshipThis research was supported by the Eskisehir Osmangazi University Research Fund with Grant no. 200811031. We are also thankful to Ece TURKMEN, the Product Manager at SEM Laboratuar Cihazlari Paz. San. Tic. A. S, (Istanbul-Turkey), who provided facilities to carry out this study.en_US
dc.identifier.doi10.1007/s11033-014-3713-5
dc.identifier.endpage8135en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue12en_US
dc.identifier.pmid25189652en_US
dc.identifier.scopus2-s2.0-84926678603en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage8127en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-014-3713-5
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8247
dc.identifier.volume41en_US
dc.identifier.wosWOS:000349005800044en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBladder canceren_US
dc.subjectmiRNAen_US
dc.subjectBioinformationen_US
dc.subjectKEGG pathwaysen_US
dc.subjectCDK4en_US
dc.subjectCDK6en_US
dc.titleInvestigation of key miRNAs and target genes in bladder cancer using miRNA profiling and bioinformatic toolsen_US
dc.typeArticleen_US

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