Molecular Characterization of Staphylococcus aureus from Clinical Sheep Mastitis Cases

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dc.contributor.authorAslantas, O.
dc.contributor.authorKeskin, O.
dc.contributor.authorYucetepe, Gullu A.
dc.date.accessioned2024-09-18T20:04:28Z
dc.date.available2024-09-18T20:04:28Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn the current study, it was aimed to examine the genes encoding toxin, biofilm and microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) of 62 Staphylococcus aureus isolated from sheep clinical mastitis cases using polvmerase chain reaction (PCR). Besides, accessory gene regulator (agr) groups and the antibiotic susceptibilities of the isolates were also determined. Most of the isolates (87.1%) were susceptible to all tested antimicrobials. The sec (38, 61.3%) and sell (50, 80.6%) were the only staphylococcal enterotoxin (SE) genes detected among the isolates. The tst gene was observed in 43 (57.3%) isolates. Whereas co-existence of sec, sell, tst was detected in 36 (48%) isolates; none of them isolates harbored eta and eth genes. However, eight (12.9%) isolates were found to be negative for SE, ET and TSST-1 genes. Hemolysin genes were detected in all isolates and the frequency of bla,hlb,hld, and hlg2 were 100%, 95.2%, 98.4%, and 61.3%, respectively. The lukED and lukM were found together in 40 (64.5%) of the isolates, and /ukED was found alone in 22 (35.5%) of the isolates. Of the biofilm-related genes, icaD was the only gene detected with a frequency of 83.9% (52). MSCRAMMs genes eno, cna, ebps, fib, fnb, clfA, and c()13 were detected in 100%, 79%, 100%, 95.2%, 100%, and 100%, respectively. None of the isolates carried the bbp gene. This study indicated that S. aureus isolates from clinical sheep mastitis possessed several virulence-associated determinants.en_US
dc.identifier.endpage105en_US
dc.identifier.issn0334-9152
dc.identifier.issn2304-8859
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85132771247en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage99en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8190
dc.identifier.volume77en_US
dc.identifier.wosWOS:000811517000012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsrael Veterinary Medical Assocen_US
dc.relation.ispartofIsrael Journal of Veterinary Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiofilmen_US
dc.subjectClinical Mastitisen_US
dc.subjectSheepen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectToxinen_US
dc.titleMolecular Characterization of Staphylococcus aureus from Clinical Sheep Mastitis Casesen_US
dc.typeArticleen_US

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