Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions

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dc.contributor.authorBayraktar, Suphi
dc.contributor.authorDuran, Nizami
dc.contributor.authorDuran, Gulay Gulbol
dc.contributor.authorEryilmaz, Naciye
dc.contributor.authorAslan, Hayat
dc.contributor.authorOnlen, Cansu
dc.contributor.authorOzer, Burcin
dc.date.accessioned2024-09-18T20:06:25Z
dc.date.available2024-09-18T20:06:25Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim: We aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (RFLP) method based on Msp I and Bln I restrictive enzyme cuts of polymerase chain reaction (PCR) products after the amplification of ITS1 and ITS2 regions of rDNA genotypically. Materials and Methods: One hundred and fifty candidal strains isolated from various clinical samples were studies/ included. Phenotypic species assessment was performed using automated VITEK-2 system and kit used with the biochemical tests. Common genomic region amplification peculiar to candidal strains was carried out using ITS1 and ITS2 primer pairs. After the amplification, PCR products were cut with Msp I and Bln I restriction enzymes for species identification. Results: The majority of Candida isolates were isolated from urine (78.6%) while other isolates were composed of strains isolated from swab, wound, blood and other samples by 11.3%, 3.3%, 2% and 4.7%, respectively. The result of RFLP analysis carried out with Msp I and Bln I restriction enzymes showed that candidal strains were Candida albicans by 45.3%, Candida glabrata by 19.3%, Candida tropicalis by 14.6%, Candida parapsilosis by 5.3%, Candida krusei by 5.3%, Candida lusitaniae by 0.6% and other candidal strains by 9.3%. Conclusion: When the ability to identify Candida to species level of phenotypic and PCR-RFLP methods was assessed, a great difference was found between these two methods. It may be argued that Msp I and Bln I restriction enzyme fragments can be used in the identification of medically important Candida species. Further studies are needed to develop this kind of restriction profile to be used in the identification of candidal strains.en_US
dc.identifier.doi10.4103/ijmm.IJMM_17_102
dc.identifier.endpage542en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.issue4en_US
dc.identifier.pmid29405146en_US
dc.identifier.scopus2-s2.0-85041697188en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage535en_US
dc.identifier.urihttps://doi.org/10.4103/ijmm.IJMM_17_102
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8511
dc.identifier.volume35en_US
dc.identifier.wosWOS:000431397200013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofIndian Journal of Medical Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCandidaen_US
dc.subjectMsp I and Bln Ien_US
dc.subjectpolymerase chain reactionen_US
dc.subjectrestriction fragment length polymorphismen_US
dc.titleIdentification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regionsen_US
dc.typeArticleen_US

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