Watermelon transformation with Zucchini yellow mosaic virus coat protein gene and comparison with parental cultivar

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dc.contributor.authorCuruk, Sebahattin
dc.contributor.authorMese, Ebru
dc.date.accessioned2024-09-18T20:11:28Z
dc.date.available2024-09-18T20:11:28Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of this work was to transfer Zucchini yellow mosaic virus coat protein (ZYMV-CP) and neomycin phosphotransferase II (NPT II) genes to the watermelon ` Crimson Sweet' (CS) genome, and to compare the transgenic progenies T1 and T2 with the nontransformed parental cultivar for morphological, pomological, growth and yield characteristics. The ZYMV-CP gene was transferred by Agrobacterium tumefaciens. The presence of the gene in transgenic T0, T1 and T2 plants was determined by polymerase chain reaction, and the results were confirmed by Southern blot. Two experiments were performed, one in the winter-spring and the other in the summer-autumn. In both experiments, the hypocotyl length of transgenic seedlings was significantly higher than that of nontransgenic parental ones. In the second experiment, the differences between transgenic and nontransgenic individuals were significant concerning fruit rind thickness, flesh firmness, fruit peduncle length, size of pistil scar, and a* values for fruit stripe or flesh color. Transferring ZYMV-CP gene to CS genome affected only a few characteristics from the 80 evaluated ones. The changes in rind thickness, flesh firmness and flesh color a* values are favorable, while the increase in the size of pistil scar is undesirable. The transgenic watermelon line having ZYMV-CP gene and the parental cultivar CS are very similar.en_US
dc.description.sponsorshipState Planning Organization (Turkey); Scientific and Technological Research Council of Turkey; Mustafa Kemal University (Antakya, Turkey)en_US
dc.description.sponsorshipTo the State Planning Organization (Turkey), the Scientific and Technological Research Council of Turkey, and the Mustafa Kemal University (Antakya, Turkey), for funding; to Prof. Rebecca Grumet and the Michigan State University, for generously providing A. tumefaciens EHA 105 with pCIB10, containing NPTII and ZYMV-CP genes, and for sharing the laboratory during the transformation experiments; and to Dr. Metin Tuna, for determination of the ploidy level of the nontransgenic control and transgenic watermelon plants by flow cytometry.en_US
dc.identifier.endpage75en_US
dc.identifier.issn0100-204X
dc.identifier.issn1678-3921
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84862234811en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage66en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8868
dc.identifier.volume47en_US
dc.identifier.wosWOS:000301584900010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherEmpresa Brasil Pesq Agropecen_US
dc.relation.ispartofPesquisa Agropecuaria Brasileiraen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCitrullus lanatusen_US
dc.subjectcomparative safety analysisen_US
dc.subjectgenetic modificationen_US
dc.subjectneomycin phosphotransferase IIen_US
dc.subjectnontransgenic watermelonen_US
dc.subjecttransgenic watermelonen_US
dc.titleWatermelon transformation with Zucchini yellow mosaic virus coat protein gene and comparison with parental cultivaren_US
dc.typeArticleen_US

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