The improvement of resistance to bacterial speck in transgenic tomato plants by Agrobacterium tumefaciens mediated transformation

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dc.contributor.authorKoc, N. K.
dc.contributor.authorKayim, M.
dc.contributor.authorYetisir, H.
dc.contributor.authorSari, N.
dc.contributor.authorYuceer, S. Unlu
dc.contributor.authorArici, S. E.
dc.date.accessioned2024-09-18T20:29:39Z
dc.date.available2024-09-18T20:29:39Z
dc.date.issued2007
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to tomato genotype Urfa-2 by the LBA4404 strain of A. tumefaciens harboring the plasmid pPTC8. The presence of nptII and pto genes in transgenic plants was proved by PCR analysis. Insertion of the pto gene into the Genome of transgenic plants and expression of the gene were confirmed by southern and northern hybridizations, respectively. The pathogen P. syringae pv. tomato was applied to all leaves of transgenic and control plants. While typical bacterial speck symptoms developed on the leaves of control plants, the transgenic plants did not display any typical symptoms of bacterial speck upon inoculation with strains 1 and 0. Some of these transgenic plants had thicker leaves than the control plants and produced abnormal flowers. The pollen of transgenic plants was used for crossing with control plants to produce F1 transgenic lines. Fruits from crossed transgenic and control plants were obtained, and F1 seeds germinated on Murashige and Skoog medium in the presence of kanamycin have developed F1 seedlings.en_US
dc.identifier.doi10.1134/S102144370701013X
dc.identifier.endpage96en_US
dc.identifier.issn1021-4437
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-33846073832en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage89en_US
dc.identifier.urihttps://doi.org/10.1134/S102144370701013X
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10996
dc.identifier.volume54en_US
dc.identifier.wosWOS:000243700900013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMaik Nauka/Interperiodica/Springeren_US
dc.relation.ispartofRussian Journal of Plant Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLycopersicon esculentumen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectPseudomonas syringae pv. tomatoen_US
dc.subjectptogeneen_US
dc.subjecttransgenic planten_US
dc.subjectbacterial specken_US
dc.titleThe improvement of resistance to bacterial speck in transgenic tomato plants by Agrobacterium tumefaciens mediated transformationen_US
dc.typeArticleen_US

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