Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze-thawed ram spermatozoa

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dc.authoridOZTURK, Ali Erdem/0000-0002-3053-4615
dc.authoridIli, Pinar/0000-0002-3107-1798
dc.authoridOzkan, Huseyin/0000-0001-5753-8985
dc.authoridBODU, Mustafa/0000-0002-8432-7347
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorAkalin, Pinar Peker
dc.contributor.authorKeskin, Nazan
dc.contributor.authorBodu, Mustafa
dc.contributor.authorOzturk, Ali Erdem
dc.contributor.authorIli, Pinar
dc.contributor.authorOzkan, Huseyin
dc.date.accessioned2024-09-18T20:20:06Z
dc.date.available2024-09-18T20:20:06Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Freeze-thawing process negatively affects ram spermatozoa in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of spermatozoa during freeze-thawing is suggested to improve sperm parameters. Objectives: The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant parameters, antioxidant-related gene expressions and DNA integrity during the freeze-thawing process, in low glycerol concentration. Methods: Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris-based extender containing different concentrations of glycerol (G5; %5 and G3; %3), fetuin (F; 2.5, 5 and 15 mg/mL) and trehalose (60 mm) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5 and G3F15). Results: G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p < 0.05). Also, G3F0 displayed considerably more cryoprotective effect compared with G5F0 group (p < 0.05) in terms of motility, mitochondrial activity and viability rates. Lipid peroxidation levels decreased in G5F5 group compared with G5F0 group (p < 0.05). The levels of total glutathione increased in G3F2.5 group (p < 0.05) in comparison with the G5F0 group. NQO1 gene levels were upregulated approximately twofold in G5F5, G5F15, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). The levels of GCLC gene were approximately twofold higher in G3F0, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). GSTP1 gene levels were significantly higher with different levels in all treatment groups except for G5F2.5 and G3F0 groups in comparison with G5F0 group (p < 0.05). Conclusions: Co-supplementation of tris-based extender having low glycerol (3%) with trehalose and fetuin to enhance the quality of ram spermatozoa after freeze-thawing process is recommended.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114O642]en_US
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 114O642).en_US
dc.identifier.doi10.1111/andr.12974
dc.identifier.endpage1009en_US
dc.identifier.issn2047-2919
dc.identifier.issn2047-2927
dc.identifier.issue3en_US
dc.identifier.pmid33438325en_US
dc.identifier.scopus2-s2.0-85100113093en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1000en_US
dc.identifier.urihttps://doi.org/10.1111/andr.12974
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10051
dc.identifier.volume9en_US
dc.identifier.wosWOS:000613671000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofAndrologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectfetuinen_US
dc.subjectfreeze-thawingen_US
dc.subjectglycerolen_US
dc.subjectram spermatozoaen_US
dc.subjecttrehaloseen_US
dc.titleCombination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze-thawed ram spermatozoaen_US
dc.typeArticleen_US

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