Detection and partial characterization of grapevine leafroll-associated virus 1 in pomegranate trees in Turkey

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dc.authoridElci, Eminur/0000-0002-6434-6321
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorElci, Eminur
dc.contributor.authorGazel, Mona
dc.date.accessioned2024-09-18T20:55:33Z
dc.date.available2024-09-18T20:55:33Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractFoliar virus-like symptoms consisting of yellowing, chlorotic spots, oak-leaf and vein clearing were observed on pomegranate cultivar Hicaz in Hatay province of Turkey in 2013. Three symptomatic out of 23 pomegranate samples reacted to Grapevine leafroll-associated virus 1 (GLRaV-1) antibodies in DAS-ELISA. In order to confirm the presence of GLRaV-1 in pomegranate, total RNA extracted from petiole samples was used in RT-PCR using specific primers designed on sequences of the heat-shock protein 70 homolog (HSP70h), coat protein (CP), coat protein duplicate 2 (CPd2) and open reading frame 9 (p24) genes of GLRaV-1. Amplicons were only obtained from symptomatic pomegranate samples for the CP, CPd2, and p24 genes but, unlike for GLRaV-1 isolates from grapevine, no amplicon was obtained for the HSP70h gene of GLRaV-1 isolates from pomegranate. The CP, CPd2 and p24 genes of GLRaV-1 from pomegranate (accession no. KP411914-KP411922) had 91-94 % nucleotide sequence identity with GLRaV-1 isolates from grapevine. Phylogenetic analyses reconstructed using the neighbor joining method showed a clustering of GLRaV-1 isolates from pomegranate and grapevine. These results suggest that pomegranate could be an alternate host for GLRaV-1.en_US
dc.identifier.doi10.1007/s10658-015-0807-4
dc.identifier.endpage202en_US
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84946149234en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage199en_US
dc.identifier.urihttps://doi.org/10.1007/s10658-015-0807-4
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11919
dc.identifier.volume145en_US
dc.identifier.wosWOS:000373994100018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Journal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPunica granatum L.en_US
dc.subjectGLRaV-1en_US
dc.subjectRT-PCRen_US
dc.subjectSequencingen_US
dc.titleDetection and partial characterization of grapevine leafroll-associated virus 1 in pomegranate trees in Turkeyen_US
dc.typeArticleen_US

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