Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma

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dc.authorscopusid56051682000
dc.authorscopusid15757444100
dc.authorscopusid6506151089
dc.authorscopusid23473166000
dc.authorscopusid7003527045
dc.authorscopusid15848283700
dc.authorscopusid35339875800
dc.contributor.authorBayraktar, E.
dc.contributor.authorIgci, M.
dc.contributor.authorErturhan, S.
dc.contributor.authorIgci, Y.Z.
dc.contributor.authorKarakok, M.
dc.contributor.authorGogebakan, B.
dc.contributor.authorUlasli, M.
dc.date.accessioned2024-09-19T15:43:44Z
dc.date.available2024-09-19T15:43:44Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim of this study was to investigate expression of bikunin at the mRNA level and screen for mutations in exon sequence in renal cell carcinoma (RCC) tissues. Materials and Methods: Total RNA and DNA were extracted from paired normal and tumor tissues of total 50 RCC (11 papillary, 8 chromophobe, 26 clear cell, and 5 other types) patients (23 females, mean age: 53.55 ± 14.17; 27 males mean age: 62.1 ± 7.92). Bikunin mRNA levels were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Mutational screening was performed by using single strand conformation polymorphism (SSCP) method and nucleotide sequence analysis. Results: There was a statistically significant decrease in the 25 (50%) of tumor tissues comparing to normal tissues in terms of mRNA levels of bikunin (Wilcoxon signed rank test, p = 0.0337). According to the classification based on subtypes of RCC; clear cell RCC samples displayed a reduced gene expression (p = 0.0148). Additionally, the patients with the age above 50 had low bikunin expression. The SNP rs80057939 spanning 4th exon of bikunin was detected in 13 tumor tissues. However, it was not statistically significant (p > 0.05). Conclusion: Decreased bikunin mRNA level in renal cells might be associated with poor prognosis of renal carcinoma. Therefore, gene constructs or exogenous administration of bikunin might be a potential adjuvant therapy for RCC treatment. Copyright © Experimental Oncology, 2014.en_US
dc.identifier.endpage111en_US
dc.identifier.issn1812-9269
dc.identifier.issue2en_US
dc.identifier.pmid24980765en_US
dc.identifier.scopus2-s2.0-84903762324en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage107en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14534
dc.identifier.volume36en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMorion LLCen_US
dc.relation.ispartofExperimental Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBikuninen_US
dc.subjectNucleotide sequence analysisen_US
dc.subjectPrognostic markeren_US
dc.subjectRenal cell carcinomaen_US
dc.subjectSemi-quantitative RT-PCRen_US
dc.titleReduced gene expression of bikunin as a prognostic marker for renal cell carcinomaen_US
dc.typeArticleen_US

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