Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design
[ N/A ]
Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Natural and Engineering Science
Access Rights
info:eu-repo/semantics/openAccess
Abstract
In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). For this, State Ease Design Expert 8.0.7.1. (Serial Number: 0021-6578) software software was used applying Optimal Design. By using data obtained, Design Expert software suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test. © 2022, Natural and Engineering Science. All rights reserved.
Description
Keywords
biosensor, Carbon paste electrode, Glucose Oxidase, polyaniline, Response Surface Methodology
Journal or Series
Natural and Engineering Sciences
WoS Q Value
Scopus Q Value
Q4
Volume
7
Issue
1
