Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design
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Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Natural and Engineering Science
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). For this, State Ease Design Expert 8.0.7.1. (Serial Number: 0021-6578) software software was used applying Optimal Design. By using data obtained, Design Expert software suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test. © 2022, Natural and Engineering Science. All rights reserved.
Açıklama
Anahtar Kelimeler
biosensor, Carbon paste electrode, Glucose Oxidase, polyaniline, Response Surface Methodology
Kaynak
Natural and Engineering Sciences
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
7
Sayı
1
