EFFECT OF EXTENDER SUPPLEMENTED WITH BORON ON POST-THAW MOTILITY, VIABILITY, DNA DAMAGE AND FERTILIZATION ABILITY OF CRYOPRESERVED BROWN TROUT (Salmo trutta macrostigma) SPERMATOZOA

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.authoridYeni, Deniz/0000-0002-9105-5677
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.contributor.authorGul, Aziz
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorYeni, Deniz
dc.contributor.authorAvdatek, Fatih
dc.date.accessioned2024-09-18T20:06:15Z
dc.date.available2024-09-18T20:06:15Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBACKGROUND: Boron has been considered as an essential nutrient for decreasing lipid peroxidation and improving antioxidant mechanism in different animal species. On the other hand, its effect on quality or DNA damage following cryopreservation process in fish sperm is still unclear. OBJECTIVE: Experiments were designed to analyse the effect of an ionic based extender supplemented with boron on post-thawed motility, viability, fertility and DNA integrity of cryopreserved brown trout (Salmo trutta macrostigma) sperm. MATERIALS AND METHODS: Sperm samples were cryopreserved with the ionic extender containing different boron concentrations (0.1, 0.2, 0.3 and 0.4 mM) using a controlled freezer at two different freezing rates (FR-I: 10 degrees C min(-1) from +4 degrees C to -40 degrees C and FR-II: 15 degrees C min(-1) from +4 degrees C to -40 degrees C). Sperm motility, viability, fertilization, eyeing and DNA fragmentations were determined in post-thawed samples. RESULTS: Freezing rate-I provided significantly higher fertilization and eyeing rates compared to freezing rate-II (p<0.05). Higher post-thaw motility (62.8 +/- 1.4%) and fertilization (75.2 +/- 0.9%) rates were obtained with the 0.4 mM boron concentration at freezing rate-I. CONCLUSION: Supplementation of the extender with boron increased fertilization and eyeing rates and also decreased DNA damages at both freezing rates.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Fund [MKU BAP-1005-M-0111]en_US
dc.description.sponsorshipThis research was funded by Mustafa Kemal University Scientific Research Fund (Project No: MKU BAP-1005-M-0111).en_US
dc.identifier.endpage283en_US
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue5en_US
dc.identifier.pmid33966065en_US
dc.identifier.scopus2-s2.0-85105687698en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage275en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8410
dc.identifier.volume40en_US
dc.identifier.wosWOS:000500206900001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCryo Lettersen_US
dc.relation.ispartofCryolettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbrown trouten_US
dc.subjectboronen_US
dc.subjectspermen_US
dc.subjectcryopreservationen_US
dc.subjectmotilityen_US
dc.subjectDNA damageen_US
dc.titleEFFECT OF EXTENDER SUPPLEMENTED WITH BORON ON POST-THAW MOTILITY, VIABILITY, DNA DAMAGE AND FERTILIZATION ABILITY OF CRYOPRESERVED BROWN TROUT (Salmo trutta macrostigma) SPERMATOZOAen_US
dc.typeArticleen_US

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