Detection and serotyping of Mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies

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dc.authoridBOSCIA, DONATO/0000-0002-4544-8680
dc.authoridVarveri, Christina/0000-0001-6317-0734
dc.authoridGhanem, Gamal/0000-0003-1702-7683
dc.authoridSavino, Vito Nicola/0000-0002-8475-7777
dc.contributor.authorMyrta, A
dc.contributor.authorDi Terlizzi, B
dc.contributor.authorBoscia, D
dc.contributor.authorÇaglayan, K
dc.contributor.authorGavriel, I
dc.contributor.authorGhanem, G
dc.contributor.authorVarveri, C
dc.date.accessioned2024-09-18T20:54:19Z
dc.date.available2024-09-18T20:54:19Z
dc.date.issued1998
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.descriptionMiddle European Meeting on Plum Pox 1998 -- JUN 29-JUL 03, 1998 -- SMOLENICE, SLOVAKIAen_US
dc.description.abstractPlum pox virus (PPV) is a major threat to the expanding Mediterranean stone fruit industry. In order to control the plum pox disease it is of utmost importance to detect early PPV foci and to identify the PPV isolates involved. A survey was therefore carried out in Albania, Cyprus, Egypt, Greece, Italy and Turkey by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with the following monoclonal antibodies (MAbs): 5B (universal), 4DG5 (PPV-D-specific), AL (PPV-M-specific), TUV and AC (PPV-C-specific), and EA24 (PPV-E1 Amar-specific). A hundred and seventy Mediterranean PPV isolates were tested for strain type. PPV-M was detected in Albania, Cyprus, Greece, Italy, and Turkey; PPV-D was detected in Albania and Italy, whereas samples with natural mixtures of both strains were found in a couple of orchards in Albania. Seven PPV isolates from apricots in two Egyptian localities were recognized only by MAb EA24. In conclusion, DAS-ELISA with a combination of the universal MAb5B and the MAbs specific to the four PPV serotypes currently known (M, D, C and El Amar) is an efficient tool for a simple, sensitive and routine detection of PPV and discrimination of its serotypes.en_US
dc.identifier.endpage253en_US
dc.identifier.issn0001-723X
dc.identifier.issue4en_US
dc.identifier.pmid10073231en_US
dc.identifier.scopus2-s2.0-0032436448en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage251en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11739
dc.identifier.volume42en_US
dc.identifier.wosWOS:000078073900015en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSlovak Academic Press Ltden_US
dc.relation.ispartofActa Virologicaen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectplum pox virusen_US
dc.subjectMediterranean isolatesen_US
dc.subjectmonoclonal antibodiesen_US
dc.subjectenzyme-linked immunosorbent assayen_US
dc.titleDetection and serotyping of Mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodiesen_US
dc.typeConference Objecten_US

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