A new and simple pathogenicity test using carrot slices forPseudomonas savastanoipv.savastanoi, causal disease agent of olive knot
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Olive knot disease caused byPseudomonas savastanoipv.savastanoiis one of the important problems in olive (Olea europaeaL), pomegranate (Punica granatumL.), myrtus (Myrtus communisL.) and jasmine (Jasminum officinaleL.) in Hatay province, Turkey. The disease agent causes young seedling mortalities in intensive infections. The pathogenicity tests on the host plant seedling takes a long time to confirm identity and virulence of isolates/strains. In this study, we focused on a new and rapid pathogenicity test method forP. s.pv.savastanoiisolated from olive, pomegranate,myrtus and jasmine plants using carrot slices. Following inoculation of carrot slices withP. s.pv.savastanoiisolates, first typical knots were observed within a week and the knots fully developed on carrot slices 14 days after inoculation. Bacterial isolates were re-isolated from carrot slices and re-identified asP. s.pv.savastanoiusing LOPAT tests, MALDI-TOF MS and molecular methods. One-year-old olive seedlings (cv. Gemlik) were inoculated with the re-isolatedP. s.pv.savastanoiMKUBK-HZP14 for confirmation of carrot slice inoculation results. Typical knot symptoms were observed 60 days after inoculation of olive seedlings. The findings showed the carrot slice method to be a very simple and rapid technique for testing pathogenicity ofP. s.pv.savastanoiisolates. The main advantage of this technique was found to be its sensitivity and promptness which yields correct results within 14 days.
Açıklama
Anahtar Kelimeler
Olive knot, Daucus carota, Identification, Virulence test
Kaynak
Journal of Plant Pathology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
102
Sayı
4
