Molecular mechanisms and genomic basis of tigecycline-resistant Enterobacterales from swine slaughterhouses

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dc.authoridRuichao, Li/0000-0001-8333-9891
dc.contributor.authorPeng, Kai
dc.contributor.authorWang, Qian
dc.contributor.authorLi, Yan
dc.contributor.authorWang, Mianzhi
dc.contributor.authorKurekci, Cemil
dc.contributor.authorLi, Ruichao
dc.contributor.authorWang, Zhiqiang
dc.date.accessioned2024-09-18T20:57:05Z
dc.date.available2024-09-18T20:57:05Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe continuous emergence of tigecycline-resistant bacteria is undermining the effectiveness of clinical tigecy-cline. Environmental tigecycline-resistant bacteria have the potential to infect humans through human -environment interactions. Furthermore, the mechanisms of tigecycline resistance in Enterobacterales are complicated. In this study, we aimed to investigate the additional pathways of tigecycline resistance in envi-ronmental Enterobacterales besides tet(X) and tmexCD-toprJ. During the years 2019-2020, tigecycline-resistant Enterobacterales (n = 45) negative for tet(X) and tmexCD-toprJ were recovered from 328 different samples from two slaughterhouses. Five distinct bacteria species were identified, of which Klebsiella pneumoniae (n = 37) was the most common, with K. pneumoniae ST45 and ST35 being the predominant clones. Tigecycline resistance determinants analysis showed that tet(A) mutations and ramR inactivation were the most prevalent mechanisms for tigecycline resistance in the 45 strains. Two known tet(A) variants (type 1 and tet(A)-v) and one novel tet(A) variant (type 3) were identified. Cloning experiments confirmed that the novel type 3 tet(A) could enhance the 4 -fold MIC for tigecycline. Inactivation of ramR was induced by either point mutations or indels of sequences, which could result in the overexpression of AcrAB pump genes leading to tigecycline resistance. In addition, all isolates were resistant to a wide range of antimicrobials and carried various resistance genes. These findings enriched the epidemiological and genomic characterizations of tigecycline-resistant Enterobacterales from slaughterhouses and contributed to a better understanding of the complex mechanisms of tigecycline resistance in environmental bacteria.en_US
dc.description.sponsorshipNational Natural Science Foundation of China [32161133005]; Jiangsu Agricultural Science and Technology Innovation Fund [CX(21)2010]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)en_US
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (no. 32161133005), Jiangsu Agricultural Science and Technology Innovation Fund CX(21)2010, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).en_US
dc.identifier.doi10.1016/j.micres.2022.127151
dc.identifier.issn0944-5013
dc.identifier.issn1618-0623
dc.identifier.pmid35985220en_US
dc.identifier.scopus2-s2.0-85135877755en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.micres.2022.127151
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12278
dc.identifier.volume264en_US
dc.identifier.wosWOS:000844310300003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofMicrobiological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSlaughterhouse environmenten_US
dc.subjectTigecycline resistanceen_US
dc.subjecttet(A)en_US
dc.subjectTransmissionen_US
dc.subjectMobile elementsen_US
dc.titleMolecular mechanisms and genomic basis of tigecycline-resistant Enterobacterales from swine slaughterhousesen_US
dc.typeArticleen_US

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