The Association of Intrauterine Antimicrobial Resistant Trueperella pyogenes with Cytological Endometrial Inflammation Status in Repeat Breeder Cows
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Zoological Soc Pakistan
Access Rights
info:eu-repo/semantics/openAccess
Abstract
This study describes the presence of Trueperella pyogenes from routine bacteriological examination, sequence analysis for antimicrobial resistance of the identified bacteria, and the association of T. pyogenes with cytological endometrial inflammation status in 32 repeat breeder cow enrolled in the study. Intrauterine samples for bacteriological examination were collected with cotton swabs; samples for subclinical endometritis were collected with cytotape technique. Antimicrobial resistance to commonly used antibiotics was evaluated by disc diffusion method. Presence of the tetW gene responsible for tetracycline resistance was detected by PCR. Subclinical endometritis, breed, parity, calving history, early postpartum uterine infection, ovarian tissue findings, and body condition score were analyzed as potential factors for T. pyogenes presence in repeat breeder cows. A total of 15 isolates were identified as T. pyogenes. These were combined to analyze the results by both biochemical and sequence analysis of the 16s rDNA gene. Subclinical endometritis was detected in 37.5% cows. The T. pyogenes strains were highly resistant (100%) to bacitracin, tetracycline, neomycin, and oxytetracycline. All tetracycline resistant T. pyogenes strains were positive for the tetW gene. The variables had no effect on the presence of T. pyogenes in the repeat breeder cows. In conclusion, intrauterine T. pyogenes presence was not associated with subclinical endometritis, although T. pyogenes was detected in the later stages of lactation.
Description
Keywords
Cow, Cytological endometritis, Repeat breeder, Trueperella pyogenes
Journal or Series
Pakistan Journal of Zoology
WoS Q Value
Q4
Scopus Q Value
Q4
Volume
54
Issue
1
