The Association of Intrauterine Antimicrobial Resistant Trueperella pyogenes with Cytological Endometrial Inflammation Status in Repeat Breeder Cows

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Küçük Resim

Tarih

2022

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Zoological Soc Pakistan

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

This study describes the presence of Trueperella pyogenes from routine bacteriological examination, sequence analysis for antimicrobial resistance of the identified bacteria, and the association of T. pyogenes with cytological endometrial inflammation status in 32 repeat breeder cow enrolled in the study. Intrauterine samples for bacteriological examination were collected with cotton swabs; samples for subclinical endometritis were collected with cytotape technique. Antimicrobial resistance to commonly used antibiotics was evaluated by disc diffusion method. Presence of the tetW gene responsible for tetracycline resistance was detected by PCR. Subclinical endometritis, breed, parity, calving history, early postpartum uterine infection, ovarian tissue findings, and body condition score were analyzed as potential factors for T. pyogenes presence in repeat breeder cows. A total of 15 isolates were identified as T. pyogenes. These were combined to analyze the results by both biochemical and sequence analysis of the 16s rDNA gene. Subclinical endometritis was detected in 37.5% cows. The T. pyogenes strains were highly resistant (100%) to bacitracin, tetracycline, neomycin, and oxytetracycline. All tetracycline resistant T. pyogenes strains were positive for the tetW gene. The variables had no effect on the presence of T. pyogenes in the repeat breeder cows. In conclusion, intrauterine T. pyogenes presence was not associated with subclinical endometritis, although T. pyogenes was detected in the later stages of lactation.

Açıklama

Anahtar Kelimeler

Cow, Cytological endometritis, Repeat breeder, Trueperella pyogenes

Kaynak

Pakistan Journal of Zoology

WoS Q Değeri

Q4

Scopus Q Değeri

Q4

Cilt

54

Sayı

1

Künye