Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity

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dc.authoridRoumi, Vahid/0000-0002-6496-2298
dc.authoridRuiz-Garcia, Ana Belen/0000-0001-5704-2138
dc.authoridReynard, Jean-Sebastien/0000-0002-2337-107X
dc.authoridCandresse, Thierry/0000-0001-9757-1835
dc.authoridTUNC, BAHAR/0000-0001-7990-7622
dc.authoridOlmos, Antonio/0000-0001-8406-7963
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorGazel, Mona
dc.contributor.authorRoumi, Vahid
dc.contributor.authorKocabag, Hamide Deniz
dc.contributor.authorTunc, Bahar
dc.contributor.authorReynard, Jean Sebastien
dc.contributor.authorRuiz-Garcia, Ana Belen
dc.date.accessioned2024-09-18T20:55:34Z
dc.date.available2024-09-18T20:55:34Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0-86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV.en_US
dc.description.sponsorshipEuropean Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [734736]; Marie Curie Actions (MSCA) [734736] Funding Source: Marie Curie Actions (MSCA)en_US
dc.description.sponsorshipThis study was supported by funds from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 734736-VirFree project.en_US
dc.identifier.doi10.3390/agronomy10111821
dc.identifier.issn2073-4395
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85109091436en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/agronomy10111821
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11921
dc.identifier.volume10en_US
dc.identifier.wosWOS:000592925300001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofAgronomy-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPunica granatumen_US
dc.subjectpotyviridaeen_US
dc.subjectHTSen_US
dc.subjectelectron microscopyen_US
dc.subjectRT-PCRen_US
dc.titleIdentification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversityen_US
dc.typeArticleen_US

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