Antimicrobial susceptibility, presence of resistance genes and biofilm formation in coagulase negative staphlococci isolated from subclinical sheep mastitis

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dc.authorscopusid22233445200
dc.authorscopusid56088920000
dc.authorscopusid6504329703
dc.authorscopusid56351947600
dc.authorscopusid56523456000
dc.authorscopusid55159723400
dc.contributor.authorErgün, Yaşar
dc.contributor.authorAslantaş, Özkan
dc.contributor.authorKireçci, Ekrem
dc.contributor.authorÖztürk, Fatma
dc.contributor.authorCeylan, Ahmet
dc.contributor.authorBoyar, Yilmaz
dc.date.accessioned2024-09-19T15:43:33Z
dc.date.available2024-09-19T15:43:33Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this study, coagulase negative staphylococci (CoNS) (n = 70) isolated from subclinical sheep mastitis were screened for minimum inhibitory concentration (MIC) to antimicrobials used commonly in veterinary field in Turkey. In addition, plasmid profiling and biofilm production of CoNS isolates was investigated. All isolates were found to be susceptible to amoxycillin-clavulanic acid, cephalothin, gentamicin, enrofloxacin and oxacillin. The highest resistance was observed in 42.9% (n = 30) of the isolates against the beta-lactam antibiotics, penicillin and ampicillin. All beta-lactam resistant isolates produced beta-lactamase and carried blaZ. Tetracycline resistance was observed in 11.4% (n = 8) of the isolates, either alone or in combination with beta-lactams and macrolides. Of the tetracycline resistant 8 isolates, 5 carried the tetK gene, one carried the tetM and 2 isolates carried both genes together. Erythromycin resistance was observed in 5.7% of the isolates; msrA was detected alone (one isolate) or in combination with mphC (one isolate) and ermC (one isolate). ermA was observed only in one isolate. Most of the strains showed only a single plasmid band in size of 19.3 kb, but some had 2 to 3 plasmids ranging from >19.3 kb to 0.9 kb. Out of 70 CoNS isolates, 28 (40%) were identified as biofilm producer by Congo red agar (CRA) method, and 30 (42.9%) were positive for both icaA and icaD genes, which are known to be responsible for biofilm formation in CoNS.en_US
dc.identifier.doi10.9775/kvfd.2011.5643
dc.identifier.endpage456en_US
dc.identifier.issn1300-6045
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84859205248en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage449en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2011.5643
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14411
dc.identifier.volume18en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherVeteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectBiofilm productionen_US
dc.subjectCoagulase negative staphylococcien_US
dc.subjectSheepen_US
dc.subjectSubclinical mastitisen_US
dc.titleAntimicrobial susceptibility, presence of resistance genes and biofilm formation in coagulase negative staphlococci isolated from subclinical sheep mastitisen_US
dc.title.alternativeSubklinik koyun mastitislerinden i?zole edilen koagulaz negatif stafilokok suşlarının antibiyotik duyarlılıkları, direnç genlerinin varlı?ı ve biyofilm sentezien_US
dc.typeArticleen_US

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