Comparison by Sequence-Based and Electron Microscopic Analyses of Fig mosaic virus Isolates Obtained from Field and Experimentally Inoculated Fig Plants

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dc.authoridcaliskan, oguzhan/0000-0002-2583-9588
dc.authoridMedina, Vicente/0000-0002-1030-2235
dc.authoridSOYLU, Soner/0000-0003-1002-8958
dc.authoridSoylu, Emine Mine/0000-0001-5961-0848
dc.authoridULUBAS SERCE, CIGDEM/0000-0001-5337-5883
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorSerce, Cigdem Ulubas
dc.contributor.authorBarutcu, Eminur
dc.contributor.authorKaya, Kamuran
dc.contributor.authorMedina, Vicente
dc.contributor.authorGazel, Mona
dc.contributor.authorSoylu, Soner
dc.date.accessioned2024-09-18T21:05:10Z
dc.date.available2024-09-18T21:05:10Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractFig mosaic disease (FMD) and the fig mite, Aceria ficus, are widespread in different fig growing provinces of Turkey. Fig trees (Ficus carica) cv. Bursa siyahi (D1) and an unknown seedling (D2) that showed typical FMD symptoms and was heavily infested by fig mites were used as donor plants for attempted mite transmissions to healthy fig seedlings. Transmission electron microscopy observations of donor plant samples prior to the transmission tests were performed and showed the presence of double membrane bodies (DMBs) in the palisade mesophyll cells. Electron microscopy of all experimentally inoculated fig seedlings showed the same bodies. This result reinforced the suggestion that an agent that elicits the production of DMBs in infected cells is involved in the etiology of FMD. Double-stranded (ds)RNA analyses were also performed from experimentally inoculated plants, and dsRNAs with sizes approximately 1.30 and 1.96 kb were obtained. Reverse transcription polymerase chain reaction (RT-PCR) products of 468 and 298 bp specific to Fig mosaic virus (FMV) were amplified from both donor and experimentally inoculated plants. BLAST analyses of nucleotide sequences of these fragments showed 90% identity with FMV for the donor plant and 94 to 96% for experimentally inoculated plants. According to these results, FMV is present in both donor and experimentally inoculated plants in Turkey, and this virus is transmissible by A. ficus from fig plant to fig plant.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK-TOAG-106O134]en_US
dc.description.sponsorshipThe authors thank Bryce W. Falk for his critical reading of the manuscript. This study was supported by a grant (number TUBITAK-TOAG-106O134) from the Scientific and Technological Research Council of Turkey.en_US
dc.identifier.doi10.1094/PDIS-11-09-0771
dc.identifier.endpage1452en_US
dc.identifier.issn0191-2917
dc.identifier.issn1943-7692
dc.identifier.issue12en_US
dc.identifier.pmid30743390en_US
dc.identifier.scopus2-s2.0-78650016156en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1448en_US
dc.identifier.urihttps://doi.org/10.1094/PDIS-11-09-0771
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13422
dc.identifier.volume94en_US
dc.identifier.wosWOS:000284447100009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Phytopathological Socen_US
dc.relation.ispartofPlant Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSense Rna Virusen_US
dc.subjectMultipartiteen_US
dc.subjectTransmissionen_US
dc.subjectDiseaseen_US
dc.subjectCytopathologyen_US
dc.subjectAgentsen_US
dc.titleComparison by Sequence-Based and Electron Microscopic Analyses of Fig mosaic virus Isolates Obtained from Field and Experimentally Inoculated Fig Plantsen_US
dc.typeArticleen_US

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