EFFECT OF DIFFERENT THAWING RATES ON MOTILITY AND FERTILIZING CAPACITY OF CRYOPRESERVED GRASS CARP (CTENOPHARYNGODON IDELLA) SPERM

Basit Öğe Kaydı
dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorYavas, Ilker
dc.contributor.authorBozkurt, Yusuf
dc.date.accessioned2024-09-18T20:25:30Z
dc.date.available2024-09-18T20:25:30Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCryopreservation of spermatozoa has been well developed in many fish species for resource conservation and aquaculture practices. There are limited data on the effect of cryopreservation on grass carp (Ctenopharyngodon idella) spermatozoa. This research was carried out to investigate the effect of different thawing procedures on motility and fertilizing capacity of frozen! thawed grass carp semen as preliminary data to design future cryopreservation experiments. Semen was collected by abdominal stripping from adult male grass carp. Collected semen was diluted with extender containing 350 mM glucose, 30 mM Tris and 5% glycerole (pH 8.0). Diluted samples were packaged in 0.25 ml straws and left to equilibrate for 30 min at4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapour for 10 min and plunged into the liquid nitrogen (-196 degrees C). For thawing, the straws were removed from liquid nitrogen and immersed in 30, 35 and 40 C water for 10, 20 and 30 seconds. In fertilization experiments spermatozoa:egg ratio was 1x10(5):1. The highest mean motility (83.4 +/- 2.1) (p<0.05) and fertilization rate (85.6 +/- 2.8) (P<0.05) was determined from semen thawed at 35 C for 30 sec. The results indicate that thawing rates significantly influenced motility and fertilizing ability of cryopreserved grass carp semen.en_US
dc.identifier.doi10.5504/BBEQ.2011.0018
dc.identifier.endpage2257en_US
dc.identifier.issn1310-2818
dc.identifier.issn1314-3530
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-79953055310en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2254en_US
dc.identifier.urihttps://doi.org/10.5504/BBEQ.2011.0018
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10335
dc.identifier.volume25en_US
dc.identifier.wosWOS:000287943500014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiotechnology & Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGrass carpen_US
dc.subjectcryopreservationen_US
dc.subjectthawing rateen_US
dc.subjectsemenen_US
dc.subjectmotilityen_US
dc.subjectfertilizationen_US
dc.titleEFFECT OF DIFFERENT THAWING RATES ON MOTILITY AND FERTILIZING CAPACITY OF CRYOPRESERVED GRASS CARP (CTENOPHARYNGODON IDELLA) SPERMen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full Text
Boyut:
1.57 MB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu