Investigation of liposome formulation effects on rivastigmine transport through human colonic adenocarcinoma cell line (CACO-2)

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dc.authorscopusid6603404070
dc.authorscopusid35798621100
dc.authorscopusid24082008800
dc.authorscopusid6505835107
dc.authorscopusid55915615100
dc.contributor.authorDe?im, Z.
dc.contributor.authorMutlu, N.B.
dc.contributor.authorYilmaz, Ş.
dc.contributor.authorEşsiz, D.
dc.contributor.authorNacar, A.
dc.date.accessioned2024-09-19T15:49:43Z
dc.date.available2024-09-19T15:49:43Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractTransportations of rivastigmine containing liposomes across Caco-2 cells were studied and in vitro test results were compared with in vivo results. MTT test was used for cell viability studies. Series of formulations were prepared containing rivastigmine which is used for the treatment of Alzheimer's disease. Characterization and stability studies for liposome formulations were performed. Encapsulation efficiencies of liposomes were 35.4%, 25.2% and 29.9% for rivastigmine, rivastigmine-sodium taurocholate, rivastigmine-dimethylbeta-CD liposomes, respectively. In stability studies, particle size and size distribution, zeta potential, rivastigmine amounts were determined and shelf lives of liposomes were calculated. Penetration properties of rivastigmine through Caco-2 cells, dialysis membrane and kinetics of release from liposomes were determined. Permeability coefficients were calculated after diffusion studies. The highest value of % cumulative amount of rivastigmine passed through caco-2 cell cultures was found to be 87.2% for rivastigmine-sodium taurocholate solution and 12.8% for rivastigmine-sodium taurocholate liposome. The highest permeability coefficient value was obtained with sodium taurocholate liposomes for -0.75. Rivastigmine liposomes and solutions were also applied to animals. Acetyl choline esterase (AChE) activity was determined by the Ellman method on mice. %AChE inhibition values were calculated using blood and brain tissue samples. The physical appearances of the brains were investigated by TEM microscope. The highest value of AChE inhibition was observed for rivastigmine and sodium taurocholate liposomes. The histological investigations and observations also supported these results.en_US
dc.identifier.doi10.1691/ph.2010.9179
dc.identifier.endpage40en_US
dc.identifier.issn0031-7144
dc.identifier.issue1en_US
dc.identifier.pmid20187576en_US
dc.identifier.scopus2-s2.0-76149095641en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage32en_US
dc.identifier.urihttps://doi.org/10.1691/ph.2010.9179
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15351
dc.identifier.volume65en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofPharmazieen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAbsorptionen_US
dc.subjectAnimalsen_US
dc.subjectbeta-Cyclodextrinsen_US
dc.subjectBiological Transport, Activeen_US
dc.subjectBrainen_US
dc.subjectCaco-2 Cellsen_US
dc.subjectCell Survivalen_US
dc.subjectChemistry, Pharmaceuticalen_US
dc.subjectCholinesterase Inhibitorsen_US
dc.subjectChromatography, High Pressure Liquiden_US
dc.subjectDrug Compoundingen_US
dc.subjectExcipientsen_US
dc.subjectHumansen_US
dc.subjectLiposomesen_US
dc.subjectMaleen_US
dc.subjectMiceen_US
dc.subjectMice, Inbred BALB Cen_US
dc.subjectMicroscopy, Electron, Transmissionen_US
dc.subjectParticle Sizeen_US
dc.subjectPhenylcarbamatesen_US
dc.subjectTaurocholic Aciden_US
dc.subjectcyclodextrin derivativeen_US
dc.subjectdimethyl beta cyclodextrinen_US
dc.subjectliposomeen_US
dc.subjectrivastigmineen_US
dc.subjecttaurocholic aciden_US
dc.subjectunclassified drugen_US
dc.subjectanimal experimenten_US
dc.subjectanimal tissueen_US
dc.subjectarticleen_US
dc.subjectcancer cell cultureen_US
dc.subjectcell strain CACO 2en_US
dc.subjectcell viabilityen_US
dc.subjectcontrolled studyen_US
dc.subjectcytotoxicityen_US
dc.subjectdrug distributionen_US
dc.subjectdrug formulationen_US
dc.subjectdrug penetrationen_US
dc.subjectdrug releaseen_US
dc.subjectdrug transporten_US
dc.subjectencapsulationen_US
dc.subjectenzyme stabilityen_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectmaleen_US
dc.subjectmouseen_US
dc.subjectnonhumanen_US
dc.subjectparticle sizeen_US
dc.subjectzeta potentialen_US
dc.titleInvestigation of liposome formulation effects on rivastigmine transport through human colonic adenocarcinoma cell line (CACO-2)en_US
dc.typeArticleen_US

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