Silver nanoparticles potentiate antitumor and oxidant actions of cisplatin via the stimulation of TRPM2 channel in glioblastoma tumor cells

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dc.authoridNAZIROGLU, Mustafa/0000-0003-0887-6974
dc.authoridakyuva, yener/0000-0001-8171-5929
dc.contributor.authorAkyuva, Yener
dc.contributor.authorNazirog, Mustafa
dc.date.accessioned2024-09-18T20:02:34Z
dc.date.available2024-09-18T20:02:34Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractWe investigated the effects of silver nanoparticle (AgNP) and cisplatin (CiSP) exposure via the activation of TRPM2 cation channels in glioblastoma (DBTRG-05MG) cell line. The cells were divided into four groups as control, AgNPs (100 mu g/ml for 48 h), CiSP (25 mu M for 24 h), and CiSP + AgNPs. We found that the cytotoxic, oxidant and apoptotic actions of CiSP were further stimulated through the activation of TRPM2 (via ADP-ribose and H2O2) in the cells by the treatment of AgNPs. The actions were decreased in the cells by the treatments of TRPM2 antagonists (ACA and 2APB). The apoptotic actions of AgNPs were induced by the stimulation of propidium iodide positive DBTRG-05MG rate, caspase -3, caspase -8, and caspase -9 activations, although their oxidant actions were acted by the increase of mitochondrial membrane depolarization, lipid peroxidation, mitochondrial oxygen free radicals (ROS), and cytosolic ROS, but the decrease of total antioxidant status, glutathione, and glutathione peroxidase. The accumulation of cytosolic free Ca2+ and Zn2+ into mitochondria via the activation of TRPM2 current density and activity accelerated oxidant and apoptotic actions of AgNPs in the cells. We found that the combination of AgNPs and CiSP was synergistic via the stimulation of TRPM2 for treatment of DBTRG-05MG cells. The combination of AgNPs and CiSP showed a favorable action via the stimulation of TRPM2 in the treatment of glioblastoma tumor cells.en_US
dc.description.sponsorshipBSN Health, Analysis and Innovation Ltd. Inc; Goller Bolgesi Teknokenti, Isparta, Turkey [2021-10]en_US
dc.description.sponsorshipThe present study was financially supported by BSN Health, Analysis and Innovation Ltd. Inc. Goller Bolgesi Teknokenti, Isparta, Turkey. (Project No: 2021-10. The owner of the project is Dr. Yener Akyuva).en_US
dc.identifier.doi10.1016/j.cbi.2022.110261
dc.identifier.issn0009-2797
dc.identifier.issn1872-7786
dc.identifier.pmid36403784en_US
dc.identifier.scopus2-s2.0-85142732776en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.cbi.2022.110261
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7873
dc.identifier.volume369en_US
dc.identifier.wosWOS:000975845700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofChemico-Biological Interactionsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectGlioblastomaen_US
dc.subjectMitochondriaen_US
dc.subjectSilver nanoparticlesen_US
dc.subjectTRPM2 channelen_US
dc.subjectZinc ionen_US
dc.titleSilver nanoparticles potentiate antitumor and oxidant actions of cisplatin via the stimulation of TRPM2 channel in glioblastoma tumor cellsen_US
dc.typeArticleen_US

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