Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey

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dc.authoridColeri Cihan, Arzu/0000-0002-7289-6251
dc.contributor.authorCihan, Arzu Coleri
dc.contributor.authorOzcan, Birgul
dc.contributor.authorTekin, Nilgun
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2024-09-18T20:28:05Z
dc.date.available2024-09-18T20:28:05Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe phylogenetic diversity of 31 thermophilic bacilli belonging to genera Geobacillus and Aeribacillus were investigated which were isolated from various geothermal sites of Turkey. Twenty-seven of these isolates were found to be belonged within the genus Geobacillus, whereas 4 of them were identified as Aeribacillus pallidus. The comparative 16S rRNA gene sequence analyses revealed that the A. pallidus isolates displayed sequence similarity values from 98.0 to 99.6% to their closest relative. Furthermore, Geobacillus isolates showed sequence similarity values from 88.9 to 99.8% with the reference type strains. According to the phylogenetic analysis, isolates belonging to genus Geobacillus were diverged into nine clusters and among these isolates, 19 of them were identified as strains related to G. caldoproteolyticus, G. thermodenitrificans, G. stearothermophilus, G. thermoglucosidasius and G. toebii with the most abundant 13 isolates from G. caldoproteolyticus. Four of the Geobacillus isolates were named as unidentified mix group, as they found to be genetically very homogenous like their closely related type species: G. thermoleovorans, G. vulcani, G. lituanicus, G. kaustophilus, G. caldovelox, G. caldotenax, and G. uralicus. Moreover, the sequence comparisons of E173a, E265, C161ab and A142 isolates demonstrated that they represented novel species among genus Geobacillus as they shared lower than 96.7% sequence similarity to all the described type species. The AluI-, HaeIII- and TaqI-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. By ARDRA results, the isolates were able to be differentiated and clustered, the discriminative restriction fragments of these isolates and type species were determined and the novelty of E173, E265, C161ab and A142 isolates could be displayed. Some differentiating phenotypic characters and the ability of amylase, glucosidase and protease production of these bacilli were also studied and biotechnologically valuable thermostable enzyme producing isolates were introduced in order to use in further studies.en_US
dc.description.sponsorshipFundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK) [TBAG/HD-320 (107T286)]en_US
dc.description.sponsorshipThis study was supported by Fundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK); project number TBAG/HD-320 (107T286). In addition we are very grateful to Prof. Dr. Hakan Akbulut (Ankara University, Faculty of Medicine, Oncology Department, Ankara, Turkey) and Associated Prof. Dr. Hilal Ozdag (Ankara University, Biotechnology Institute Central Laboratory, Genomic Unite, Ankara, Turkey) for their contributions.en_US
dc.identifier.doi10.1007/s11274-011-0742-2
dc.identifier.endpage2696en_US
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85027928009en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2683en_US
dc.identifier.urihttps://doi.org/10.1007/s11274-011-0742-2
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10724
dc.identifier.volume27en_US
dc.identifier.wosWOS:000295506000025en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWorld Journal of Microbiology & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIsolationen_US
dc.subjectThermophilicen_US
dc.subjectGeobacillusen_US
dc.subjectAeribacillusen_US
dc.subject16S rRNA geneen_US
dc.subjectARDRAen_US
dc.titlePhylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkeyen_US
dc.typeArticleen_US

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