Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm

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dc.authoridAkcay, Ergun/0000-0002-7491-5671
dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Y
dc.contributor.authorAkcay, E
dc.contributor.authorTekin, N
dc.contributor.authorSecer, S
dc.date.accessioned2024-09-18T20:13:34Z
dc.date.available2024-09-18T20:13:34Z
dc.date.issued2005
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractRainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in their own extenders at 30 degrees C for 30 s. For both freezing methods, the best fertilization results were obtained with extender III containing 15% DMSO, 52.3% (56.3% of the control) for sperm frozen in straws and 48.4% (52.4% of the control) for sperm frozen as pellets. The interaction between extender and cryoprotectant was statistically significant (p<0.05).en_US
dc.identifier.endpage130en_US
dc.identifier.issn0792-156X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-22144481347en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage125en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9268
dc.identifier.volume57en_US
dc.identifier.wosWOS:000230557600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsraeli Journal Of Aquaculture-Bamidgehen_US
dc.relation.ispartofIsraeli Journal of Aquaculture-Bamidgehen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcryopreservationen_US
dc.subjectextenderen_US
dc.subjectfertilizationen_US
dc.subjectOncorhynchus mykissen_US
dc.subjectpelleten_US
dc.subjectspermen_US
dc.subjectstrawen_US
dc.titleEffect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) spermen_US
dc.typeArticleen_US

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