Effect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissue

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dc.authoridMcKerlie, Colin/0000-0002-2232-0967
dc.contributor.authorYildiz, Cengiz
dc.contributor.authorMullen, Brendan
dc.contributor.authorJarvi, Keith
dc.contributor.authorMcKerlie, Colin
dc.contributor.authorLo, Kirk C.
dc.date.accessioned2024-09-18T20:32:58Z
dc.date.available2024-09-18T20:32:58Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractRestoration of male fertility associated with use of the cryopreserved testicular tissue would be a significant advance in human and animal assisted reproductive technology. The purpose of this study was to test the effects of four different cryoprotectant agents (CPA) on spermatogenesis and steroidogenesis in cryopreserved and allotransplanted neonatal mouse testicular tissue. Hank's balanced salt solution (HBSS) with 5% fetal bovine serum including either 0.7 M dimethyl sulfoxide (DMSO), 0.7 M propylene glycol (PrOH), 0.7 M ethylene glycol (EG), or glycerol was used as the cryoprotectant solution. Donor testes were collected and dissected from neonatal pups of CD-1 mice (one day old). Freezing and seeding of the testicular whole tissues was performed using an automated controlled-rate freezer. Four fresh (non-frozen) or frozen-thawed pieces of testes were subcutaneously grafted onto the hind flank of each castrated male NCr nude recipient mouse and harvested after 3 months. Fresh neonatal testes grafts recovered from transplant sites had the most advanced rate of spermatogenesis with elongated spermatid and spermatozoa in 46.6% of seminiferous tubules and had higher levels of serum testosterone compared to all other frozen-thawed-graft groups (p < 0.05). Fresh grafts and frozen-thawed grafts in the DMSO group had the highest rate of tissue survival compared to PrOH, EG, and glycerol after harvesting (p > 0.05). The most effective CPA for the freezing and thawing of neonatal mouse testes was DMSO in comparison with EG (p < 0.05) in both pre-grafted and post-grafted tissues based on histopathological evaluation. Likewise, the highest level of serum testosterone was obtained from the DMSO CPA group compared to all other cryoprotectants evaluated (p < 0.05). The typical damage observed in the frozen-thawed grafts included disruption of the interstitial stroma, intercellular connection ruptures, and detachment of spermatogonia from the basement membrane. These findings indicate that neonatal mouse testes were most effectively preserved when frozen with HBSS medium with DMSO and that the type of CPA is a significant factor to obtain the most advanced stages of spermatogenesis and steroidogenesis after cryopreservation, thawing, and transplantation of neonatal mouse testes. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipCanadian Urological Associationen_US
dc.description.sponsorshipThis project is funded by the Canadian Urological Association Scholarship Fund.en_US
dc.identifier.doi10.1016/j.cryobiol.2013.05.004
dc.identifier.endpage75en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue1en_US
dc.identifier.pmid23721968en_US
dc.identifier.scopus2-s2.0-84879551647en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2013.05.004
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11242
dc.identifier.volume67en_US
dc.identifier.wosWOS:000321601600011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMouse testesen_US
dc.subjectSpermatogenesisen_US
dc.subjectSpermatozoaen_US
dc.subjectGraftingen_US
dc.subjectCryopreservationen_US
dc.subjectTestosteroneen_US
dc.titleEffect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissueen_US
dc.typeArticleen_US

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