Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri

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dc.authoridKucukgul, Azime/0000-0002-0515-6667
dc.authoridGonenci, Ramazan/0000-0002-4043-2612
dc.contributor.authorKucukgul, Azime
dc.contributor.authorKucukgul, Altug
dc.contributor.authorGonenci, Ramazan
dc.contributor.authorOzsoy, Sule Yurdagul
dc.contributor.authorKutlu, Banu
dc.contributor.authorIsgor, Mehmet Mustafa
dc.date.accessioned2024-09-18T20:02:34Z
dc.date.available2024-09-18T20:02:34Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractYersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri-infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) and bacteria (1.5x10(8)cfu/mL) were determined by viability tests. The expression of caspases 1, 3, 8, and 12 was determined by real-time PCR and protein levels investigated by ELISA method. Apoptotic-necrotic cell ratios were determined by acridine orange and ethidium bromide staining. Bacterial stimulations of caspases (1, 3, 8, and 12) were suppressed by ozone mostly at the translational level. While bacterial infection increased the count of apoptotic and necrotic cells at different stages, ozone significantly reduced this condition. Obtained data indicate possible use of nontoxic ozone as a safe and effective treatment alternative for increasing host defenses and eliminating the infection in the case of yersiniosis in fish.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkeyen_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey supported this research. We thank Prof. Dr. Christopher Secombes (The University of Aberdeen/Scotland) for providing RTS-11 (monocyte/macrophage cell line).en_US
dc.identifier.doi10.1007/s10499-019-00364-8
dc.identifier.endpage783en_US
dc.identifier.issn0967-6120
dc.identifier.issn1573-143X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85064051523en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage771en_US
dc.identifier.urihttps://doi.org/10.1007/s10499-019-00364-8
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7870
dc.identifier.volume27en_US
dc.identifier.wosWOS:000466854000011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAquaculture Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCaspasesen_US
dc.subjectNontoxic ozone mixtureen_US
dc.subjectProtein levelsen_US
dc.subjectYersinia ruckerien_US
dc.titleInvestigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckerien_US
dc.typeArticleen_US

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