Comparison of different freezing techniques, extenders, and cryoprotectants on quality and fertility of cryopreserved Salmo trutta f. fario sperm

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.date.accessioned2024-09-18T20:06:15Z
dc.date.available2024-09-18T20:06:15Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThere is still a lack of standardized methodology in the cryopreservation of Salmo trutta f. fario sperm. Thus, the present study was designed to compare current freezing protocols to improve and as well as to examine the post-thaw quality and fertilizing ability of cryopreserved sperm in Salmo trutta f. fario. Sperm samples were diluted at a 1:10 ratio in one of three extenders (Alsever's solution, glucose-based, and ionic-based) containing four types of cryoprotectant (DMSO, DMA, MeOH, glycerol) at 10% concentration and frozen in 0.1 mL pellets on surface of the dry ice (solid carbon dioxide, 79 degrees C) or in 0.25 mL straws 2 cm above of the liquid nitrogen (LN2) surface at a rate of similar to 30 degrees C for 10 min(-1) before storage in a mL cryotank (-196 degrees C). The frozen sperm cells in straws and pellets were thawed in a water bath at 25 degrees C for 30 s and at 20 degrees C for 6 s respectively. Fertilization was carried out using a ratio of 5x10(5) sperm/egg in both freezing methods. The glucose-based solution including glycerol produced the highest post-thaw progressive motility (62.5 +/- 1.24%), motility duration (57.2 +/- 0.46 s), and viability (56.4 +/- 1.57%) (p < 005) in the straw method. In breeding trials, similarly, sperm frozen-thawed with the glucose-based solution including glycerol produced the highest fertilization (54.2 +/- 0.36%) and hatching (30.6 +/- 0.28%) in the straw method. Fresh sperm used as control produced 82.6 +/- 0.45% and 78.4 +/- 1.27% fertilization and hatching respectively. It was concluded that sperm frozen in straws produced higher post-thaw sperm motility and fertility of eggs than those frozen in pellets. Also, the results suggest using of glycerol-supplemented glucose solution because of producing better results in both freezing techniques.en_US
dc.description.sponsorshipIskenderun Technical University Scientific Research Fund [ISTE BAP-2017-YP-04]en_US
dc.description.sponsorshipThis research was partially funded by Iskenderun Technical University Scientific Research Fund (Project No: ISTE BAP-2017-YP-04).en_US
dc.identifier.doi10.4025/actascitechnol.v46i1.64924
dc.identifier.issn1806-2563
dc.identifier.issn1807-8664
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85185688099en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.4025/actascitechnol.v46i1.64924
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8411
dc.identifier.volume46en_US
dc.identifier.wosWOS:001207710300024en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Estadual Maringa, Pro-Reitoria Pesquisa Pos-Graduacaoen_US
dc.relation.ispartofActa Scientiarum-Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbrown trouten_US
dc.subjectcryopreservationen_US
dc.subjectextenderen_US
dc.subjectstrawen_US
dc.subjectpelleten_US
dc.titleComparison of different freezing techniques, extenders, and cryoprotectants on quality and fertility of cryopreserved Salmo trutta f. fario spermen_US
dc.typeArticleen_US

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