Transformation of Tobacco with ScMTII Gene-Enhanced Cadmium and Zinc Accumulation

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dc.authoridDAGHAN, HATICE/0000-0002-0150-5882
dc.authoridUYGUR, VELI/0000-0003-3971-7714
dc.authoridArslan, Mehmet/0000-0002-0530-157X
dc.contributor.authorDaghan, Hatice
dc.contributor.authorArslan, Mehmet
dc.contributor.authorUygur, Veli
dc.contributor.authorKoleli, Nurcan
dc.date.accessioned2024-09-18T20:27:54Z
dc.date.available2024-09-18T20:27:54Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractGenetic transformation is gaining importance for developing plant types suitable to metal accumulate and/or hyperaccumulate. In this study, the transgenic tobacco plant which transferred the ScMTII gene from Saccharomyces cerevisiae to wild type tobacco cultivar Petite Havana (SR1) was grown on soils with low and high cadmium (Cd) and zinc (Zn) concentrations in a growth chamber for 6 weeks and compared to wild type tobacco for Cd and Zn accumulation. Cadmium and Zn accumulations in the transgenic and wild type tobacco plants were increased with the increasing Cd and Zn concentrations. Unlike Zn, the transgenic plant accumulated significantly higher amount of Cd compared to the wild type control plants. Shoot Cd concentrations of transgenic tobacco in higher Cd dosages reached the above the hyperaccumulation threshold value of 100mgCdkg1 in the dry weight (DW). Transgenic tobacco accumulated 354, 400, 372, and 457mgCdkg1 DW, for 10, 20, 40, and 80mgCdkg1 soil treatments, respectively. These values are 3.54.5-fold higher than that of Cd hyperaccumulation threshold value. With 10mgkg1 Cd treatment, the bioconcentration factor (BCF) of transgenic tobacco plants for Cd reached up to 35 in which the threshold value for BCF should be at least 10. Our results showed that the transgenic tobacco may be used as a good Cd hyperaccumulator plant and for phytoextraction of Cd contaminated soils, but not for Zn.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [108-O-161]en_US
dc.description.sponsorshipWe would like to thank Prof. Rainer Fisher for supplying tobacco plants material. This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) as a research project (Project No: 108-O-161). The authors thank Prof. M. N. V. Prasad (University of Hyderabad, India) for his kind English revision of the text.en_US
dc.identifier.doi10.1002/clen.201200298
dc.identifier.endpage509en_US
dc.identifier.issn1863-0650
dc.identifier.issn1863-0669
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84876428764en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage503en_US
dc.identifier.urihttps://doi.org/10.1002/clen.201200298
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10592
dc.identifier.volume41en_US
dc.identifier.wosWOS:000317866600014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofClean-Soil Air Wateren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHyperaccumulator planten_US
dc.subjectMetallothioneinen_US
dc.subjectNicotiana tabacumen_US
dc.subjectPhytoextractionen_US
dc.subjectTransgenic tobaccoen_US
dc.titleTransformation of Tobacco with ScMTII Gene-Enhanced Cadmium and Zinc Accumulationen_US
dc.typeArticleen_US

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