Impact of Enoxaparin +? Lipoic acid combination on oxidative stress, follicle development and apoptotic damage in ovarian ischemia reperfusion model

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dc.authorscopusid56369756800
dc.authorscopusid36926064000
dc.authorscopusid22937913600
dc.authorscopusid56387061800
dc.authorscopusid57210586313
dc.authorscopusid57641043900
dc.contributor.authorOzcil, M. Dogan
dc.contributor.authorOzcan, O.
dc.contributor.authorHakverdi, S.
dc.contributor.authorBayraktar, H.S.
dc.contributor.authorDirican, E.
dc.contributor.authorKacmaz, F.
dc.date.accessioned2024-09-19T15:43:36Z
dc.date.available2024-09-19T15:43:36Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractOBJECTIVE: With this study we aimed at investigating the impact of enoxaparin (E) and ?-lipoic acid (?-LA) on ischemia/reperfusion (I/R) damage in an experimental ovarian torsion (T) detorsion (D) model. MATERIALS AND METHODS: The study comprised 80 female albino rats aged 12 weeks. They were divided into 8 groups as follows, with 10 rats in each group: Group 1 (Sham), Group 2 (Ischemia, 3 hours), Group 3 (I/R, 6 hours reperfusion), Group 4 (I/R, 3 days reperfusion), Group 5 (I/R, E, 6 hours), Group 6 (I/R, E, 3 days), Group 7 (I/R, E+?-LA, 6 hours), and Group 8 (I/R, E+?-LA, 3 days). Immediately after detorsion, enoxaparin (0.5 mg/kg/day, sc) or ?-LA (100 mg/kg/day, ip) were administered. Ovarian levels of malondialdehyde (MDA), glutathione peroxidase (GPx), and catalase were measured. In addition to total Antioxidant Status (TAS) and Total Oxidative Stress (TOS), the Oxidative Stress Index (OSI) was calculated. Serum levels of AMH, FSH, LH, E2, and progesterone were measured. Ovarian samples were histopathologically examined and they underwent Tag staining for apoptosis. RESULTS: Compared with the sham group, there was a significant increase in TOS, OSI, and MDA levels in I/R group (p<0.001). Compared to Group 2, a significant increase was determined in TOS and OSI in Group 3 (p<0.05). In Group 4, a significant increase was found compared to Group 2 (p<0.01). The lowest level of I/R damage and the highest level of antioxidants were found in Group 2, 7, and 8. The highest values of TOS, OSI, and MDA were found in Group 3, Group 4, Group 5, and Group 6 (p<0.001). When the sham group and Group 4 were compared, it was found that AMH and E2 levels decreased (p<0.001), while FSH levels increased (p<0.001). In Group 6, AMH and E2 values were significantly decreased compared to the sham group (p<0.001). In the histopathological examination, the findings were observed to be parallel to those of the biochemical markers. CONCLUSIONS: Combined use of ?-Lipoic acid and enoxaparin prevents oxidative stress and ovarian damage due to ischemia-reperfusion injury better than the use of drugs alone. © 2022 Verduci Editore s.r.l. All rights reserved.en_US
dc.identifier.doi10.26355/eurrev_202209_29758
dc.identifier.endpage6592en_US
dc.identifier.issn1128-3602
dc.identifier.issue18en_US
dc.identifier.pmid36196708en_US
dc.identifier.scopus2-s2.0-85139572320en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage6583en_US
dc.identifier.urihttps://doi.org/10.26355/eurrev_202209_29758
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14448
dc.identifier.volume26en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVerduci Editore s.r.len_US
dc.relation.ispartofEuropean Review for Medical and Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectEnoxaparinen_US
dc.subjectIschemia/ eperfusionen_US
dc.subjectOvarian torsionen_US
dc.subjectOxidative stressen_US
dc.subject?-Lipoicen_US
dc.titleImpact of Enoxaparin +? Lipoic acid combination on oxidative stress, follicle development and apoptotic damage in ovarian ischemia reperfusion modelen_US
dc.typeArticleen_US

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