Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility

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dc.contributor.authorGul, Aziz
dc.contributor.authorSahinler, Nuray
dc.contributor.authorOnal, Ali G.
dc.contributor.authorHopkins, Brandon K.
dc.contributor.authorSheppard, Walter S.
dc.date.accessioned2024-09-18T20:26:46Z
dc.date.available2024-09-18T20:26:46Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different extenders added post-thaw on the fertilization rate of cryopreserved honey bee semen. The purpose of adding extender post-thaw was to dilute the cryoprotectant to remove chemicals after centrifugation because of potential adverse effects. The control consisted of frozen-thawed semen without the addition of an extender; treatment groups included the addition of one of the following extenders: glucose solution, fresh ram semen plasma, fresh honey bee semen plasma, extender solution. All of the above treatments and frozen-thawed control were compared to fresh semen. For each group, 15 virgin queens were instrumentally inseminated with the semen-diluent solution and introduced into nucleus colonies to determine the brood patterns of the queens. Percentages of worker brood produced in the fresh semen, frozen-thawed semen control, glucose, fresh ram semen plasma, fresh honey bee semen plasma, and extender solution supplemented groups were 98 +/- 1.1%, 47 +/- 0.9%, 3.0 +/- 0.8%, 0.3 +/- 0.1%, 48.1 +/- 4.1% and 40.3 +/- 2.4%, respectively. Similiarly, spermatozoa numbers in the spermathecae of the same treatment groups were 3.6 x 10(6),1.6 x 10(6), 73 x 10(6), 4.7 x 10(6), 8.1 x 10(6), and 4.6 x 10(6) spermatozoa for the same treatment, respectively. The differences in both worker brood percentage and sperm count in the spermatheca were statistically significant (P < 0.01) among all treatment groups, except the frozen thawed control group and fresh drone semen plasma group. We found a positive correlation between sperm count in the spermatheca and the percentage of worker brood (r = 0.91). With the exception of fresh honey bee semen plasma, the fertility rate was reduced following the addition of various plasmas and diluents post-freezing. (C) 2017 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey), Ankara, Turkey [1120840]en_US
dc.description.sponsorshipThis study was financially supported by the TUBITAK (The Scientific and Technological Research Council of Turkey) [Project no: 1120840], Ankara, Turkey.en_US
dc.identifier.doi10.1016/j.theriogenology.2017.06.020
dc.identifier.endpage113en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid28708507en_US
dc.identifier.scopus2-s2.0-85021409532en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2017.06.020
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10528
dc.identifier.volume101en_US
dc.identifier.wosWOS:000407187800013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHoney beeen_US
dc.subjectCryopreservationen_US
dc.subjectInstrumental inseminationen_US
dc.subjectLiquid nitrogenen_US
dc.titleEffects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertilityen_US
dc.typeArticleen_US

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