Glucose-6-phosphate dehydrogenase, glutathione peroxidase, total glutatione and reduced nicotinamide adenine dinucleotide phosphate in milk cells of subclinical mastitic cows

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dc.authoridBastan, Ayhan/0000-0001-8291-1147
dc.contributor.authorAkalin, P. P.
dc.contributor.authorErgun, Y.
dc.contributor.authorBaspinar, N.
dc.contributor.authorDogruer, G.
dc.contributor.authorKucukgul, A.
dc.contributor.authorCantekin, Z.
dc.contributor.authorIsgor, M.
dc.date.accessioned2024-09-18T20:59:10Z
dc.date.available2024-09-18T20:59:10Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was collected and grouped by the California Mastitis Test. Then, a somatic cell count (SCC) was performed, and the groups were re-scored as control (5-87 x 10(3) cells), 1st group (154-381 x 10(3) cells), 2nd group (418-851 x 10(3) cells), 3rd group (914-1958 x 10(3) cells), and 4th group (2275-8528 x 10(3) cells). Milk cell TP, NADPH, tGSH levels, G6PD, and GPx activities were assessed. Microbiological diagnosis and aerobic mesophyle general organism (AMG, cfu/g) were also conducted. In mastitic milk, TP, NADPH, and tGSH levels, and G6PD and GPx activities were significantly reduced per cell (in samples of 10(6) cells). In addition, milk SCC was positively correlated with AMG (r=0.561, p<0.001), NADPH (r=0.380, p<0.01), TP (r=0.347, p<0.01) and G6PD (r=0.540, p<0.001). There was also positive correlation between NADPH (r=0.428, p<0.01), TP (r=0.638, p<0.001) and AMG. NADPH was positively correlated with TP (r=0.239, p<0.05), GPx (r=0.265, p<0.05) and G6PD (r=0.248, p=0.056). Total protein was positively correlated with tGSH (r=0.354, p<0.01) and G6PD (r=0.643, p<0.001). There was a negative correlation between tGSH and GPx activity (r=-0.306, p<0.05). The microbiological analysis showed the following ratio of pathogens: Coagulase-Negative Staphylococci 66.6%, Streptococcus spp 9.5%, Bacillus spp 9.5%, yeast 4.8%, and mixed infections 9.5%. As a conclusion, when evaluating the enzyme and oxidative stress parameters in milk, it is more suitable to assign values based on cell count rather than ml of milk. The linear correlation between the SCC and AMG, milk cell NADPH, TP and G6PD suggests that these parameters could be used as markers of mastitis.en_US
dc.description.sponsorshipScientific Research Coordinating Office (BAP) of Mustafa Kemal University [12161]en_US
dc.description.sponsorshipThis study was supported by Scientific Research Coordinating Office (BAP) of Mustafa Kemal University (Project No. 12161).en_US
dc.identifier.doi10.24425/pjvs.2019.129216
dc.identifier.endpage278en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue2en_US
dc.identifier.pmid31269353en_US
dc.identifier.scopus2-s2.0-85069269005en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage271en_US
dc.identifier.urihttps://doi.org/10.24425/pjvs.2019.129216
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12422
dc.identifier.volume22en_US
dc.identifier.wosWOS:000473738800011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztynen_US
dc.relation.ispartofPolish Journal of Veterinary Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectsubclinical mastitisen_US
dc.subjectmilk cellen_US
dc.subjectglucose-6-phosphate dehydrogenaseen_US
dc.subjectglutathione peroxidaseen_US
dc.subjectNADPHen_US
dc.titleGlucose-6-phosphate dehydrogenase, glutathione peroxidase, total glutatione and reduced nicotinamide adenine dinucleotide phosphate in milk cells of subclinical mastitic cowsen_US
dc.typeArticleen_US

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