Preliminary Studies on the Detection of Phytoplasmas in Cherry by Microscopy Techniques

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dc.contributor.authorSertkaya, G.
dc.date.accessioned2024-09-18T20:55:31Z
dc.date.available2024-09-18T20:55:31Z
dc.date.issued2008
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description5th International Cherry Symposium -- JUN 06-10, 2005 -- Bursa, TURKEYen_US
dc.description.abstractSome symptoms consisting of growth reduction, leaf chlorosis, thin shoots with wilted leaves, die-back and decline have been observed on sweet cherry (Prunus avium L.) trees in the Eastern Mediterranean Region of Turkey in many years. Symptomatic plants were inspected in the field. Shoot and leaf samples were collected in 2004 from affected orchard trees in Adana in early spring and autumn for detection of phytoplasma infections by fluorescence and electron microscopy techniques. Small pieces of stems from symptomatic plants were stained with 4'-6-diamidino-2-phenylindole (DAPI) and examined with an epifluorescence microscope. Ultra-thin sections of midribs were examined under a transmission electron microscope (TEM). A total of 11 samples collected in late summer were tested with both techniques. Three samples reacted positive by DAPI. Only one DAPI-positive sample was detected by TEM analyses. Demonstration of the presence of phytoplasma-like bodies in sieve tubes was not easy because many of them collapsed. No typical fluorescence was detected in sieve tubes of shoot samples taken from the same trees in early spring. Although the number of positive samples was not high among the detected trees, according to symptoms observed and to fluorescence microscopy results, the causal agent is suggested to be a phytoplasma. Results obtained from TEM analyses confirmed the presence of phytoplasma-like bodies in sieve tubes of the samples. This is the first report of phytoplasma in cherry trees in Turkey based on microscopy techniques. Studies on the detection of phytoplasmas by molecular techniques are in progress. Further investigations also are necessary to gain more knowledge of the distribution and natural transmission of the related agent(s) in sour (P. cerasus L.) and sweet cherry varieties in Turkey.en_US
dc.description.sponsorshipISHS Sect Pome & Stone Fruits,ISHS WG Cherry Product,Int Soc Horticultural Sci,Uludag Univ,Sci & Technol Res Council Turkey,Ataturk Cent Horticultural Res Inst Turkey,Turkish Soc Horticultural Scien_US
dc.identifier.doi10.17660/ActaHortic.2008.795.151
dc.identifier.endpage937en_US
dc.identifier.isbn978-90-6605-551-3
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.issue795en_US
dc.identifier.scopus2-s2.0-58049198354en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage933en_US
dc.identifier.urihttps://doi.org/10.17660/ActaHortic.2008.795.151
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11885
dc.identifier.wosWOS:000260482900151en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInt Soc Horticultural Scienceen_US
dc.relation.ispartofProceedings of The Vth International Cherry Symposium, Vols 1 and 2en_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPrunus aviumen_US
dc.subjectsweet cherryen_US
dc.subjectfluorescence and transmission electron microscopyen_US
dc.subjectDAPIen_US
dc.titlePreliminary Studies on the Detection of Phytoplasmas in Cherry by Microscopy Techniquesen_US
dc.typeConference Objecten_US

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