Detection and Identification of Phytoplasmas in Pomegranate Trees with Yellows Symptoms

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dc.authoridCONTALDO, NICOLETTA/0000-0003-2398-4850
dc.authoridBertaccini, Assunta/0000-0002-5650-1512
dc.contributor.authorGazel, Mona
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorBaspinar, Huseyin
dc.contributor.authorMejia, Juan F.
dc.contributor.authorPaltrinieri, Samanta
dc.contributor.authorBertaccini, Assunta
dc.contributor.authorContaldo, Nicoletta
dc.date.accessioned2024-09-18T20:54:15Z
dc.date.available2024-09-18T20:54:15Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractSymptoms resembling those associated with phytoplasma presence were observed in pomegranate (Punica granatum L.) trees in June 2012 in the Aegean Region of Turkey (Aydn province). The trees exhibiting yellowing, reduced vigour, deformations and reddening of the leaves and die-back symptoms were analysed to verify phytoplasma presence. Total nucleic acids were extracted from fresh leaf midribs and phloem tissue from young branches of ten symptomatic and five asymptomatic plants. Nested polymerase chain reaction assays using universal phytoplasma-specific 16S rRNA and tuf gene primers were performed. Amplicons were digested with Tru1I, Tsp509I and HhaI restriction enzymes, according to the primer pair employed. The phytoplasma profiles were identical to each other and to aster yellows (16SrI-B) strain when digestion was carried out on 16Sr(I)F1/R1 amplicons. However, one of the samples showed mixed profiles indicating that 16SrI-B and 16SrXII-A phytoplasmas were present when M1/M2 amplicons were digested, the reamplification of this sample with tuf cocktail primers allowed to verify the presence of a 16SrXII-A profile. One pomegranate aster yellows strain AY-PG from 16S rRNA gene and the 16SrXII-A amplicon from tuf gene designed strain STOL-PG were directly sequenced and deposited in GenBank under the Accession Numbers KJ818293 and KP161063, respectively. To our knowledge, this is the first report of 16SrI-B and 16SrXII-A phytoplasmas in pomegranate trees.en_US
dc.identifier.doi10.1111/jph.12401
dc.identifier.endpage140en_US
dc.identifier.issn0931-1785
dc.identifier.issn1439-0434
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84954025870en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage136en_US
dc.identifier.urihttps://doi.org/10.1111/jph.12401
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11704
dc.identifier.volume164en_US
dc.identifier.wosWOS:000367835300009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Phytopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectphytoplasmaen_US
dc.subjectpomegranateen_US
dc.titleDetection and Identification of Phytoplasmas in Pomegranate Trees with Yellows Symptomsen_US
dc.typeArticleen_US

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