Simultaneous and sequential co-immobilization of glucose oxidase and catalase onto florisil

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dc.authoridTukel, Seyhan/0000-0003-2503-2781
dc.contributor.authorOzyilmaz, Gul
dc.contributor.authorTukel, S. Seyhan
dc.date.accessioned2024-09-18T20:25:25Z
dc.date.available2024-09-18T20:25:25Z
dc.date.issued2007
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe co-immobilization of Aspergillus niger glucose oxidase (GOD) with bovine liver catalase (CAT) onto florisil (magnesium silicate-based porous carrier) was investigated to improve the catalytic efficiency of GOD against H2O2 inactivation. The effect of the amount of bound CAT on the GOD activity was also studied for 12 different initial combinations of GOD and CAT, using simultaneous and sequential coupling. The sequentially co-immobilized GOD-CAT showed a higher efficiency than the simultaneously co-immobilized GOD-CAT in terms of the GOD activity and economic costs. The highest activity was shown by the sequentially co-immobilized GOD-CAT when the initial amounts of GOD and CAT were 10 mg and 5 mg per gram of carrier. The optimum pH, buffer concentration, and temperature for GOD activity for the same co-immobilized GOD-CAT sample were then determined as pH 6.5, 50 mM, and 30 degrees C, respectively. When compared with the individually immobilized GOD, the catalytic activity of the co-immobilized GOD-CAT was 70% higher, plus the reusability was more than two-fold. The storage stability of the co-immobilized GOD-CAT was also found to be higher than that of the free form at both 5 degrees C and 25 degrees C. The increased GOD activity and reusability resulting from the co-immobilization process may have been due to CAT protecting GOD from inactivation by H2O2 and supplying additional O-2 to the reaction system.en_US
dc.identifier.endpage967en_US
dc.identifier.issn1017-7825
dc.identifier.issn1738-8872
dc.identifier.issue6en_US
dc.identifier.pmid18050914en_US
dc.identifier.scopus2-s2.0-34547395935en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage960en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10290
dc.identifier.volume17en_US
dc.identifier.wosWOS:000247703500011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Soc Microbiology & Biotechnologyen_US
dc.relation.ispartofJournal of Microbiology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectglucose oxidaseen_US
dc.subjectcatalaseen_US
dc.subjectsimultaneous coimmobilizationen_US
dc.subjectsequential co-immobilizationen_US
dc.subjectflorisilen_US
dc.titleSimultaneous and sequential co-immobilization of glucose oxidase and catalase onto florisilen_US
dc.typeArticleen_US

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