The effect of spacer arm on hydrolytic and synthetic activity of Candida rugosa lipase immobilized on silica gel

Basit Öğe Kaydı
dc.contributor.authorOzyilmaz, Gul
dc.date.accessioned2024-09-18T20:29:35Z
dc.date.available2024-09-18T20:29:35Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCandida rugosa lipase was covalently immobilized onto silica gel in two different ways: via glutaraldehyde (L-GAL) and via hydrophobic spacer arm (1,6 diamino hexane) (L-SA). Free lipase, L-GAL and L-SA were used to investigate the hydrolysis of two different substrates, namely p-nitrophenyl palmytate (pNPP) and p-nitrophenyl acetate (pNPA), both in aqueous medium. In addition, these lipase samples were used to synthesize the pNPP from p-nitrophenol (pNP) and palmytic acid (PA) and pNPA from pNP and acetic acid (AA), both in hexane medium. Hydrolytic and synthetic activities of L-SA were higher than those of free lipase and LGAL, Synthetic activities of free lipase, LGAL and LSA for pNPA in the presence of pNP and AA within hexane medium were higher than those of hydrolytic activities for pNPA in aqueous medium. The same tendency was also observed with pNPP. The effects of pH and temperature on hydrolytic and synthetic activities were investigated for all lipase preparations. Operational stability was the highest for L-GAL, and L-SA when these enzymes were used for pNPP synthesis and in hexane medium, after 100 repeated uses, 68% and 51% of initial activities remained, respectively, at the end of 100 repeated cycles. Free lipase lost all of its activity within 15 and 20 days when stored at 25 degrees C and 5 degrees C, respectively. However. L-GAL showed 54% and 70% of initial activity at the end of 60 storage days at 25 degrees C and 5 degrees C, respectively. while these values were observed as 36% and 60% for L-SA. (C) 2008 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.molcatb.2008.05.008
dc.identifier.endpage236en_US
dc.identifier.issn1381-1177
dc.identifier.issn1873-3158
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-58249084182en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage231en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2008.05.008
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10953
dc.identifier.volume56en_US
dc.identifier.wosWOS:000264669400008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Catalysis B-Enzymaticen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLipaseen_US
dc.subjectImmobilizationen_US
dc.subjectSpacer armen_US
dc.subjectSynthetic activityen_US
dc.subjectHydrolytic activityen_US
dc.titleThe effect of spacer arm on hydrolytic and synthetic activity of Candida rugosa lipase immobilized on silica gelen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ N/A ]
İsim:
Tam Metin / Full Text
Boyut:
453.92 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu