Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)

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dc.authoridKucukgul, Azime/0000-0002-0515-6667
dc.contributor.authorKucukgul, A.
dc.contributor.authorGulsafak, I
dc.date.accessioned2024-09-18T20:08:04Z
dc.date.available2024-09-18T20:08:04Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractTo investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71 +/- 1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 mu g ml(-1)) except in the control group and only carvacrol (100 mu g ml(-1) diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1 beta (IL-1 beta), interferon gamma (INF-gamma), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1 beta expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-gamma expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1 beta and IFN-gamma compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1 beta and IFN-gamma expressions increased in +CAR group, expect IFN-gamma in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.en_US
dc.description.sponsorshipTunceli University Scientific Research Projects (TUNIBAP-TURKEY)en_US
dc.description.sponsorshipWe would like to thank TUNIBAP for this study supported by Tunceli University Scientific Research Projects (TUNIBAP-TURKEY). We would like to thank the Department of Biochemistry, Veterinary Faculty of Mustafa Kemal University, for helping us with the laboratory analyses.en_US
dc.identifier.doi10.22092/ijfs.2018.117382
dc.identifier.endpage214en_US
dc.identifier.issn1562-2916
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85064878104en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage205en_US
dc.identifier.urihttps://doi.org/10.22092/ijfs.2018.117382
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8595
dc.identifier.volume18en_US
dc.identifier.wosWOS:000461362300001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIranian Fisheries Science Research Inst-Ifsrien_US
dc.relation.ispartofIranian Journal of Fisheries Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarvacrolen_US
dc.subjectCaspasesen_US
dc.subjectInflammatory cytokinesen_US
dc.subjectLPSen_US
dc.subjectRainbow trouten_US
dc.titleBio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)en_US
dc.typeArticleen_US

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