Protective effects of thymoquinone against methotrexate-induced testicular injury

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dc.authoridOktar, Suleyman/0000-0003-0151-5981
dc.authoridYonden, Zafer/0000-0003-0708-5417
dc.contributor.authorGokce, Ahmet
dc.contributor.authorOktar, Suleyman
dc.contributor.authorKoc, Ahmet
dc.contributor.authorYonden, Zafer
dc.date.accessioned2024-09-18T20:59:20Z
dc.date.available2024-09-18T20:59:20Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThymoquinone is the major active component derived from Nigella sativa. Methotrexate is a folic acid antagonist widely used in clinic. Aim of this study was to investigate the possible protective role of thymoquinone on testicular toxicity of methotrexate. Experiments were performed on male C57BL/6 mice (6 weeks old, 20 +/- 2 g). The animals were divided into four groups with six mice in each group. Equivalent volumes of saline were injected intraperitoneally (i.p.) in the control group. In the thymoquinone group, mice received thymoquinone i.p. with a dose of 10 mg/kg/day for 4 days. Mice in the methotrexate group received single dose of methotrexate i.p., with a dose of 20 mg/kg. Finally, in the methotrexate plus thymoquinone group, in the first and the following 3 days after methotrexate administration, thymoquinone was injected with a dose of 10 mg/kg/day, i.p. At the end of the experiment, the left testis was quickly removed and divided into two parts for histological examination and biochemical analysis. Methotrexate alone increased total antioxidant capacity and myeloperoxidase activity compared to the controls. Thymoquinone treatment decreased total antioxidant capacity and prevented the increase in the myeloperoxidase activity. Light microscopy showed in mice that receiving methotrexate resulted in interstitial space dilatation, edema, severe disruption of the seminiferous epithelium and reduced diameter of the seminiferous tubules. Administration of thymoquinone reversed histological changes of methotrexate significantly. We suggest that thymoquinone use may decrease the destructive effects of methotrexate on testicular tissue of patients using this agent.en_US
dc.identifier.doi10.1177/0960327110382564
dc.identifier.endpage903en_US
dc.identifier.issn0960-3271
dc.identifier.issue8en_US
dc.identifier.pmid20813795en_US
dc.identifier.scopus2-s2.0-79960759529en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage897en_US
dc.identifier.urihttps://doi.org/10.1177/0960327110382564
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12520
dc.identifier.volume30en_US
dc.identifier.wosWOS:000292889800010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecttestesen_US
dc.subjectthymoquinoneen_US
dc.subjectmethotrexateen_US
dc.subjectoxidative stressen_US
dc.titleProtective effects of thymoquinone against methotrexate-induced testicular injuryen_US
dc.typeArticleen_US

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