The comparison of changes in fecal and mucosal microbiome in metabolic endotoxemia induced by a high-fat diet

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dc.contributor.authorSagkan-Ozturk, Aliye
dc.contributor.authorArpaci, Abdullah
dc.date.accessioned2024-09-18T20:32:46Z
dc.date.available2024-09-18T20:32:46Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of this study was to compare the mucosal and fecal microbiota in a high fat diet-induced metabolic endotoxemia (ME) model and to identify potential species that represent dysbiosis and might mediate the inflammatory process. Fourteen male wistar albino rats were fed a standard diet (n = 7) and a high-fat diet (HFD) (n = 7). The standard diet (2600 kcal/kg) contained 3% of energy from fat and HFD (6740 kcal/kg) contained 67% beef tallow. After feeding for 12 weeks, all rats were sacrificed after fasting for 12 h and blood samples were collected. Fresh faecal samples and descending colon samples of rats were collected in sterile plastic tubes using a clean technique, immediately snap-frozen in liquid nitrogen, and then stored at -80 degrees C until used for analysis. Serum glucose, TRG, TLR4, LPS, and fecal LPS increased in the HFD group. On the contrary, HDL was higher and statistically significant in the CD group. The levels of IL-6 and TNF-alpha in the colon tissue of the HFD group were significant. The HFD group caused a significant increase in LPS levels in serum and feces. In addition, the gut and mucosal microbiome were positively/negatively correlated with the ME markers (IL-6, TNF alpha LPS). The results showed that gut and mucosal microbiome changes were associated with HFD. These changes were dense at species levels. The current study demonstrated changes in gut and mucosal microbiota in HFD-induced metabolic endotoxemia. (c) 2022 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Projects Coordinating Office of Hatay, Turkey; Hatay Mustafa Kemal University, Turkey [18.D.014]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordinating Office of Hatay, Turkey; Hatay Mustafa Kemal University, Turkey (Project number: 18.D.014).en_US
dc.identifier.doi10.1016/j.anaerobe.2022.102615
dc.identifier.issn1075-9964
dc.identifier.issn1095-8274
dc.identifier.pmid35850456en_US
dc.identifier.urihttps://doi.org/10.1016/j.anaerobe.2022.102615
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11102
dc.identifier.volume77en_US
dc.identifier.wosWOS:001167775300001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofAnaerobeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMetabolic endotoxemiaen_US
dc.subjectHigh fat dieten_US
dc.subjectMicrobiotaen_US
dc.subjectFecesen_US
dc.subjectMucosaen_US
dc.titleThe comparison of changes in fecal and mucosal microbiome in metabolic endotoxemia induced by a high-fat dieten_US
dc.typeArticleen_US

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