Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey

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dc.authoridRigling, Daniel/0000-0002-4338-5364
dc.authoridULUBAS SERCE, CIGDEM/0000-0001-5337-5883
dc.authoridAKILLI SIMSEK, SECIL/0000-0002-5055-1391
dc.contributor.authorAkilli, Secil
dc.contributor.authorSerce, Cigdem Ulubas
dc.contributor.authorKatircioglu, Yakup Zekai
dc.contributor.authorMaden, Salih
dc.contributor.authorRigling, Daniel
dc.date.accessioned2024-09-18T20:13:35Z
dc.date.available2024-09-18T20:13:35Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractChestnut blight caused by the introduced fungus Cryphonectria parasitica has been responsible for the decline of Castanea sativa in Turkey since the 1960s. In this study, 72 C. parasitica isolates were recovered from the Marmara and Black Sea regions of Turkey showing white or cream-coloured culture morphology and were subjected to various tests to determine if they were infected by Cryphonectria hypovirus 1 (CHV-1). The vast majority of the isolates (69 out of 72) were vc type EU-1. Both mating types were found among a subsample of the isolates. The hypovirus was detected in 55 isolates by dsRNA extraction and/or virus specific RT-PCR on total RNA extracts. All but one isolates showed no or only weak phenol oxidase activity on agar medium containing tannic acid, typical of CHV-1 infected isolates. Through sequencing of a specific region of the hypovirus genome, we found that 24 hypovirus isolates belonged to the CHV-1 subtype I and six to the CHV-1 subtype F2. The distribution of the two CHV-1 subtypes in Turkey showed a clear geographic pattern. CHV-1 subtype I was only detected in the Marmara and western Black Sea region, whereas subtype F2 was restricted to the eastern part of the Black Sea region. The effectiveness of 23 hypovirulent isolates was tested against a virulent isolate on 2-3 years old chestnut sprouts. Ten hypovirulent isolates, all infected by CHV-1 subtype I, prevented canker development by more than 80 % suggesting that they might be suitable for biological control of chestnut blight in Turkey.en_US
dc.description.sponsorshipAnkara University; Swiss National Science Foundation (SCOPES) [IZ73Z0-127922]; Swiss National Science Foundation (SNF) [IZ73Z0_127922] Funding Source: Swiss National Science Foundation (SNF)en_US
dc.description.sponsorshipWe thank Esther Jung for her help in sequencing of the hypovirus isolates, Ali Ugur Ozcan for preparation of the map, and Simone Prospero for a critical review of the manuscript. This project was supported by the Ankara University Research Fund and the Swiss National Science Foundation (SCOPES project IZ73Z0-127922).en_US
dc.identifier.doi10.1007/s10658-012-0089-z
dc.identifier.endpage334en_US
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84871935224en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage323en_US
dc.identifier.urihttps://doi.org/10.1007/s10658-012-0089-z
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9272
dc.identifier.volume135en_US
dc.identifier.wosWOS:000313163400010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Journal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryphonectria hypovirusen_US
dc.subjectCHV-1en_US
dc.subjectRT-PCR detectionen_US
dc.subjectSequence variationen_US
dc.subjectBiological controlen_US
dc.subjectCastanea sativaen_US
dc.titleCharacterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkeyen_US
dc.typeArticleen_US

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