Regeneration in vitro from the hypocotyl of Cucumis species produces almost exclusively diploid shoots, and does not require light
[ N/A ]
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
American Society for Horticultural Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Hypocotyl explants of three cultivars of melon (Cucumis melo L.) (cvs. Revigal, Topmark and Kirkagac), and a cucumber (C. sativus L. cv. Taoz) rapidly directly regenerated multiple shoots on Murashige and Skoog medium augmented with 4.4 ?M benzyladenine. Regeneration from the hypocotyl resulted in nearly 100% diploid shoots, whereas regeneration from the cotyledons resulted in 40% to 70% polyploid regenerants. Regeneration from cotyledon explants of melon cv. Revigal required light, whereas regeneration from hypocotyl explants of melon cv. Revigal occurred in both light and darkness. Direct regeneration also occurred from the hypocotyl of cucumber cv. Taoz in both light and darkness, even though cotyledonary explants did not regenerate buds or shoots under the same conditions. This is the first report of regeneration from the Cucumis genus producing a fully diploid plant population.
Açıklama
Anahtar Kelimeler
Cotyledon, Cucumber, Melon, Photocontrol, Tetraploid
Kaynak
HortScience
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
38
Sayı
1
