Isolations of ?-glucosidase-producing thermophilic bacilli from hot springs of Turkey

dc.authoridakcelik, Nefise/0000-0001-5541-1681
dc.contributor.authorColeri, A.
dc.contributor.authorCokmus, C.
dc.contributor.authorOzcan, B.
dc.contributor.authorAkkoc, N.
dc.contributor.authorAkcelik, M.
dc.date.accessioned2024-09-18T20:28:11Z
dc.date.available2024-09-18T20:28:11Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractFrom 42 different hot springs in six provinces belonging to distinct geographical regions of Turkey, 451 thermophilic bacilli were isolated and 67 isolates with a high amylase activity were selected to determine the alpha-glucosidase production capacities by using pNPG as a substrate. alpha-Glucosidase production capacities of the isolates varied within the range from 77.18 to 0.001 U/g. Eleven of our thermophilic bacilli produced alpha-glucosidase at significant levels comparable with that of the reference strains tested; thus, five strains, F84b (77.18 U/g), A333 (48.64 U/g), F84a (36.64 U/g), E134 (32.09 U/g), and A343 (10.79 U/g), were selected for further experiments. 16S rDNA sequence analysis revealed that these selected isolates all belonged to thermophilic bacilli 16S rDNA genetic group 5, four of them representing the genus Geobacillus, while strain A343 had an uncultured bacterium as the closest relative. Changes in alpha-glucosidase levels in the intracellular and extracellular fractions were determined during 48-h cultivation of A333, A343, F84a, F84b, E134, and the reference strain G. stearothermophilus ATCC 12980. According to alpha-glucosidase production type and enzyme levels in intracellular and extracellular fractions, Geobacillus spp. A333, F84a, and F84b were defined as extracellular enzyme producers, whereas the thermophilic bacterium A343 was found to be an intracellular alpha-glucosidase producer, similar to ATCC 12980 strain. Geobacillus sp. E134 differed in alpha-glucosidase production type from all tested isolates and the reference strain; it was described as a membrane-associated cell-bound enzyme producer. In this study, apart from screening a great number of new thermophilic bacilli from the hot springs of Turkey, which have not yet been thoroughly studied, five new thermostable alpha-1,4-glucosidase-producing bacilli that have biotechnological potential with alpha-glucosidases located at different cell positions were obtained.en_US
dc.description.sponsorshipFundamental Research Group of the Scientific and Technological Research Council of Turkey (TUBITAK) [TBAG-HD126(105T039)]en_US
dc.description.sponsorshipWe are grateful to, Cagla TUKEL for 16S rDNA sequence analysis (Department of Medical Microbiology and Immunology School of Medicine, University of California at Davis, CA, USA). This research was financed by the Fundamental Research Group of the Scientific and Technological Research Council of Turkey (TUBITAK) as a fast support project with the project number TBAG-HD126(105T039).en_US
dc.identifier.doi10.1134/S0026261709010081
dc.identifier.endpage66en_US
dc.identifier.issn0026-2617
dc.identifier.issn1608-3237
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-61349141945en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage56en_US
dc.identifier.urihttps://doi.org/10.1134/S0026261709010081
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10779
dc.identifier.volume78en_US
dc.identifier.wosWOS:000263673900008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMaik Nauka/Interperiodica/Springeren_US
dc.relation.ispartofMicrobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectisolationen_US
dc.subjectthermophilic bacillien_US
dc.subject16S rDNAen_US
dc.subjectalpha-glucosidaseen_US
dc.titleIsolations of ?-glucosidase-producing thermophilic bacilli from hot springs of Turkeyen_US
dc.typeArticleen_US

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